Enhanced production of ligninolytic enzymes by ganoderma lucidum IBL-06 using lignocellulosic agricultural wastes

被引：0

作者：

Asgher M. ^{[1
]}

Sharif Y. ^{[1
]}

Bhatti H.N. ^{[1
]}

机构：

[1] University of Agriculture, Faisalabad

来源：

International Journal of Chemical Reactor Engineering | 2010年 / 8卷

关键词：

Ganoderma lucidumIBL-06; Ligninolytic enzymes; Lignocellulosic substrates; Mediators; Solid state fermentation;

D O I：

10.2202/1542-6580.2203

中图分类号：

学科分类号：

摘要：

An indigenous novel strain of Ganoderma lucidum IBL-06 was investigated for the production of ligninolytic enzymes including lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase using different lignocellulosic substrates in still culture solid-state fermentation (SSF). The fermentation flasks were inoculated and incubated at 35°C for 14 days. Samples were harvested after every 48 h to study the profile of ligninolytic enzymes produced by the fungus on different substrates. Maximum enzyme activities were noted on 10th day of incubation on rice straw. Ganoderma lucidum IBL-06 produced highest activities of lignin peroxidase (LiP) among the lignolytic enzymes. By optimizing the SSF process, maximum activities of LiP (2185 IU/ml), MnP (1972 IU/ml) and laccase (338 IU/ml) were achieved after three days incubation of rice straw at pH 4.5; temperature, 35°C; moisture, 75% and inoculum size, 6 ml, using fructose as carbon source, urea as nitrogen source, Tween-80 as surfactant and veratryl alcohol as mediator. © 2010 The Berkeley Electronic Press. All rights reserved.

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