Improving low water activity and desiccation tolerance of the biocontrol agent Pantoea agglomerans CPA-2 by osmotic treatments

被引：0

作者：

Teixidó, N. ^{[1
,2
]}

Cañamás, T.P. ^{[1
]}

Abadias, M. ^{[1
]}

Usall, J. ^{[1
]}

Solsona, C. ^{[1
]}

Casals, C. ^{[1
]}

Viñas, I. ^{[1
]}

机构：

[1] Postharvest Unit, CeRTA, Centre UdL-IRTA, Lleida, Catalonia, Spain

[2] Centre UdL-IRTA, 191 Rovira Roure Avenue, 25198 Lleida, Catalonia, Spain

来源：

Journal of Applied Microbiology | 2006年 / 101卷 / 04期

关键词：

Aims: To study the improvement of tolerance to low water activity (a w) and desiccation during spray drying in Pantoea agglomerans cells subjected to mild osmotic stress during growth. Methods and Results: The micro-organism was cultured in an unmodified liquid (control) or in a w-modified media; and viability of these cells was evaluated on unstressed (0.995) and 0.96 aw stressed solid media; in order to check total viability and aw stress tolerance respectively. Significant improvements in viability on unmodified medium were observed with cells grown for 24 h in NaCl 0.98 aw; glycerol 0.98 aw and 0.97 a w and for 48 h in NaCl 0.98 aw and 0.97 aw modified media. Both yield improvements and water stress tolerance were achieved with low aw media. Cells grown for 24 h in NaCl 0.98 aw or for 48 h in NaCl 0.98 aw; 0.97 aw and 0.96 a w; glucose 0.97 aw and glycerol 0.97 aw showed improved aw stress tolerance in comparison with control cells. The best results were obtained with NaCl treatments (0.98 aw and 0.97 aw) which also exhibited better survival rates than control cells during spray-drying process and maintained their efficacy against postharvest fungal pathogens in apples and oranges. Conclusions: NaCl treatments are very appropriate for improving P. agglomerans low aw tolerance obtaining high production levels and maintaining biocontrol efficacy. Significance and Impact of the Study: Improving stress tolerance of biocontrol agents could be an efficient way to obtain consistency and maintain efficacy of biological control under practical conditions. © 2006 The Authors;

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摘要：

Journal article (JA)

引用

页码：927 / 937

共 24 条

[21] Preventive and curative activity of combined treatments of sodium carbonates and Pantoea agglomerans CPA-2 to control postharvest green mold of citrus fruit
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POSTHARVEST BIOLOGY AND TECHNOLOGY, 2008, 50 (01) : 1 - 7
[22] Control of postharvest diseases on citrus fruit by preharvest applications of biocontrol agent Pantoea agglomerans CPA-2 part II.: Effectiveness of different cell formulations
Canamas, Teresa P.
Vinas, Immaculada
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Torres, Rosario
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POSTHARVEST BIOLOGY AND TECHNOLOGY, 2008, 49 (01) : 96 - 106
[23] Osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent Pantoea agglomerans EPS125
Bonaterra, A
Camps, J
Montesinos, E
FEMS MICROBIOLOGY LETTERS, 2005, 250 (01) : 1 - 8
[24] Control of postharvest diseases on citrus fruit by preharvest application of the biocontrol agent Pantoea agglomerans CPA-2 part I.: Study of different formulation strategies to improve survival of cells in unfavourable environmental conditions
Canamas, Teresa Paula
Vinas, Immaculada
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POSTHARVEST BIOLOGY AND TECHNOLOGY, 2008, 49 (01) : 86 - 95

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