Improving low water activity and desiccation tolerance of the biocontrol agent Pantoea agglomerans CPA-2 by osmotic treatments

被引：0

作者：

Teixidó, N. ^{[1
,2
]}

Cañamás, T.P. ^{[1
]}

Abadias, M. ^{[1
]}

Usall, J. ^{[1
]}

Solsona, C. ^{[1
]}

Casals, C. ^{[1
]}

Viñas, I. ^{[1
]}

机构：

[1] Postharvest Unit, CeRTA, Centre UdL-IRTA, Lleida, Catalonia, Spain

[2] Centre UdL-IRTA, 191 Rovira Roure Avenue, 25198 Lleida, Catalonia, Spain

来源：

Journal of Applied Microbiology | 2006年 / 101卷 / 04期

关键词：

Aims: To study the improvement of tolerance to low water activity (a w) and desiccation during spray drying in Pantoea agglomerans cells subjected to mild osmotic stress during growth. Methods and Results: The micro-organism was cultured in an unmodified liquid (control) or in a w-modified media; and viability of these cells was evaluated on unstressed (0.995) and 0.96 aw stressed solid media; in order to check total viability and aw stress tolerance respectively. Significant improvements in viability on unmodified medium were observed with cells grown for 24 h in NaCl 0.98 aw; glycerol 0.98 aw and 0.97 a w and for 48 h in NaCl 0.98 aw and 0.97 aw modified media. Both yield improvements and water stress tolerance were achieved with low aw media. Cells grown for 24 h in NaCl 0.98 aw or for 48 h in NaCl 0.98 aw; 0.97 aw and 0.96 a w; glucose 0.97 aw and glycerol 0.97 aw showed improved aw stress tolerance in comparison with control cells. The best results were obtained with NaCl treatments (0.98 aw and 0.97 aw) which also exhibited better survival rates than control cells during spray-drying process and maintained their efficacy against postharvest fungal pathogens in apples and oranges. Conclusions: NaCl treatments are very appropriate for improving P. agglomerans low aw tolerance obtaining high production levels and maintaining biocontrol efficacy. Significance and Impact of the Study: Improving stress tolerance of biocontrol agents could be an efficient way to obtain consistency and maintain efficacy of biological control under practical conditions. © 2006 The Authors;

D O I：

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摘要：

Journal article (JA)

引用

页码：927 / 937

共 24 条

[1] Improving low water activity and desiccation tolerance of the biocontrol agent Pantoea agglomerans CPA-2 by osmotic treatments
Teixido, N.
Canamas, T. P.
Abadias, M.
Usall, J.
Solsona, C.
Casals, C.
Vinas, I.
JOURNAL OF APPLIED MICROBIOLOGY, 2006, 101 (04) : 927 - 937
[2] Water activity, temperature, and pH effects on growth of the biocontrol agent Pantoea agglomerans CPA-2
Costa, E
Usall, J
Teixidó, N
Delgado, J
Viñas, I
CANADIAN JOURNAL OF MICROBIOLOGY, 2002, 48 (12) : 1082 - 1088
[3] Molecular tools applied to identify and quantify the biocontrol agent Pantoea agglomerans CPA-2 in postharvest treatments on oranges
Soto-Munoz, L.
Teixido, N.
Usall, J.
Vinas, I.
Abadias, M.
Torres, R.
POSTHARVEST BIOLOGY AND TECHNOLOGY, 2015, 100 : 151 - 159
[4] Detection and quantification by PCR assay of the biocontrol agent Pantoea agglomerans CPA-2 on apples
Soto-Munoz, Lourdes
Teixido, Neus
Usall, Josep
Vinas, Inmaculada
Torres, Rosario
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2014, 175 : 45 - 52
[5] Environmental monitoring of the biocontrol agent Pantoea agglomerans CPA-2 applied to citrus fruit at preharvest
Soto-Munoz, L.
Torres, R.
Usall, J.
Vinas, I.
Dashevskaya, S.
Teixido, N.
ANNALS OF APPLIED BIOLOGY, 2015, 167 (02) : 250 - 261
[6] The effect of nitrogen and carbon sources on growth of the biocontrol agent Pantoea agglomerans strain CPA-2
Costa, E
Teixidó, N
Usall, J
Atarés, E
Viñas, I
LETTERS IN APPLIED MICROBIOLOGY, 2002, 35 (02) : 117 - 120
[7] Acid tolerance response induced in the biocontrol agent Pantoea agglomerans CPA-2 and effect on its survival ability in acidic environments
Canamas, T. P.
Vinas, I.
Abadias, M.
Usall, J.
Torres, R.
Teixido, N.
MICROBIOLOGICAL RESEARCH, 2009, 164 (04) : 438 - 450
[8] Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and by-products
Costa, E
Teixidó, N
Usall, J
Atarés, E
Viñas, I
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 56 (3-4) : 367 - 371
[9] Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and by-products
E. Costa
N. Teixidó
J. Usall
E. Atarés
I. Viñas
Applied Microbiology and Biotechnology, 2001, 56 : 367 - 371
[10] Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2
Nunes, Carla
Bajji, Mohammed
Stepien, Valerie
Manso, Teresa
Torres, Rosario
Usall, Josep
Jijakli, M. Haissam
POSTHARVEST BIOLOGY AND TECHNOLOGY, 2008, 47 (03) : 422 - 428

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