Real-time analysis of liquid jet sample delivery stability for an X-ray free-electron laser using machine vision

被引：0

作者：

Patel, Jaydeep ^{[1
]}

Round, Adam ^{[2
]}

de Wijn, Raphael ^{[2
]}

Vakili, Mohammad ^{[2
,3
]}

Giovanetti, Gabriele ^{[2
]}

Monrroy Vilan e Melo, Diogo Filipe ^{[2
]}

E, Juncheng ^{[2
]}

Sikorski, Marcin ^{[2
]}

Koliyadu, Jayanth ^{[2
]}

Koua, Faisal H. M. ^{[2
]}

Sato, Tokushi ^{[2
]}

Mancuso, Adrian ^{[1
,2
,4
]}

Peele, Andrew ^{[1
,5
]}

Abbey, Brian ^{[1
,6
]}

机构：

[1] La Trobe Univ, Sch Comp Engn & Math Sci, Melbourne, Vic, Australia

[2] European XFEL, Schenefeld, Germany

[3] Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany

[4] Diamond Light Source, Didcot, Oxon, England

[5] Australian Nucl Sci & Technol Org ANSTO, Australian Synchrotron, Clayton, Vic, Australia

[6] La Trobe Univ, La Trobe Inst Mol Sci LIMS, Melbourne, Vic, Australia

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2024年 / 57卷

基金：

澳大利亚研究理事会;

关键词：

liquid sample delivery; image processing; serial crystallography; machine vision algorithms; X-ray free-electron lasers; SERIAL; DYNAMICS;

D O I：

10.1107/S1600576724009853

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Automated evaluation of optical microscopy images of liquid jets, commonly used for sample delivery at X-ray free-electron lasers (XFELs), enables real-time tracking of the jet position and liquid jet hit rates, defined here as the proportion of XFEL pulses intersecting with the liquid jet. This method utilizes machine vision for preprocessing, feature extraction, segmentation and jet detection as well as tracking to extract key physical characteristics (such as the jet angle) from optical microscopy images captured during experiments. To determine the effectiveness of these tools in monitoring jet stability and enhancing sample delivery efficiency, we conducted XFEL experiments with various sample compositions (pure water, buffer and buffer with crystals), nozzle designs and jetting conditions. We integrated our real-time analysis algorithm into the Karabo control system at the European XFEL. The results indicate that the algorithm performs well in monitoring the jet angle and provides a quantitative characterization of liquid jet stability through optical image analysis conducted during experiments.

引用

页码：1859 / 1870

页数：12

共 30 条

[1] Barends T. R. M., Stauch B., Cherezov V., Schlichting I., Nat. Rev. Methods Primers, 2, (2022)
[2] Berntsen P., Hadian Jazi M., Kusel M., Martin A. V., Ericsson T., Call M. J., Trenker R., Roque F. G., Darmanin C., Abbey B., Rev. Sci. Instrum, 90, (2019)
[3] Beyerer J., Puente Leon F., Frese C., Machine Vision. Automated Visual Inspection: Theory, Practice and Applications, (2016)
[4] Beyerlein K. R., Adriano L., Heymann M., Kirian R., Knos?ka J., Wilde F., Chapman H. N., Bajt S., Rev. Sci. Instrum, 86, (2015)
[5] Chapman H. N., Fromme P., Barty A., White T. A., Kirian R. A., Aquila A., Hunter M. S., Schulz J., DePonte D. P., Weierstall U., Doak R. B., Maia F. R. N. C., Martin A. V., Schlichting I., Lomb L., Coppola N., Shoeman R. L., Epp S. W., Hartmann R., Rolles D., Rudenko A., Foucar L., Kimmel N., Weidenspointner G., Holl P., Liang M., Barthelmess M., Caleman C., Boutet S., Bogan M. J., Krzywinski J., Bostedt C., Bajt S., Gumprecht L., Rudek B., Erk B., Schmidt C., Homke A., Reich C., Pietschner D.,
[6] Cheng R. K. Y., Crystals, 10, (2020)
[7] Decking W., Abeghyan S., Abramian P., Abramsky A., Aguirre A., Albrecht C., Alou P., Altarelli M., Altmann P., Amyan K., Anashin V., Apostolov E., Appel K., Auguste D., Ayvazyan V., Baark S., Babies F., Baboi N., Bak P., Balandin V., Baldinger R., Baranasic B., Barbanotti S., Belikov O., Belokurov V., Belova L., Belyakov V., Berry S., Bertucci M., Beutner B., Block A., Blocher M., Bockmann T., Bohm C., Bohnert M., Bondar V., Bondarchuk E., Bonezzi M., Borowiec P., Bosch C., Bosenberg U., Bosott
[8] Hauf S., Heisen B., Aplin S., Beg M., Bergemann M., Bondar V., Boukhelef D., Danilevsky C., Ehsan W., Essenov S., Fabbri R., Flucke G., Fulla Marsa D., Gories D., Giovanetti G., Hickin D., Jarosiewicz T., Kamil E., Khakhulin D., Klimovskaia, Kluyver T., Kirienko Y., Kuhn M., Maia L., Mamchyk D., Mariani V., Mekinda L., Michelat T., Munnich A., Padee A., Parenti A., Santos H., Silenzi A., Teichmann M., Weger K., Wiggins J., Wrona K., Xu C., Youngman C., Zhu J., Fangohr H., Brockhauser S., J. Syn
[9] Henkel A., Maracke J., Munke A., Galchenkova M., Rahmani Mashhour A., Reinke P., Domaracky M., Fleckenstein H., Hakanpaa J., Meyer J., Tolstikova A., Carnis J., Middendorf P., Gelisio L., Yefanov O., Chapman H. N., Oberthur D., Acta Cryst, A78, (2022)
[10] Kim E. S., Yoon M., IEEE Trans. Nucl. Sci, 56, pp. 3597-3606, (2009)

← 1 2 3 →