Optimization of Medium Composition and Culture Conditions of Lactobacillus plantarum NCU137

In this study， the optimal medium composition and static culture conditions of Lactiplantibacillus plantarum NCU137 were determined by single factor experiment， Plackett-Burman， steepest climb and central composite design using the number of viable bacteria as the index. The results showed that the optimal medium components were maltose 26.73 g/L， yeast extract 21.8 g/L， soybean peptone 16.59 g/L， MgSO4·7H2O 0.15 g/L， MnSO4·H2O 0.06 g/L， adenine 0.13 g/L， phenylalanine 0.051 g/L， and Tween 80 1 mL/L. The optimal culture conditions were as follows: initial pH 7.0， inoculum quantity 3%， culture temperature 30 ℃. The final viable bacteria count reached 9.2×109 CFU/mL， which laid a theoretical foundation for the high-density culture of Lactobacillus plantarium NCU137 and the preparation of high-activity bacteria. © 2022 Chinese Institute of Food Science and Technology. All rights reserved.