Ultrasound-assisted extraction and antioxidative activities of polysaccharides from oriental tobacco buds

被引：0

作者：

Technology Center, China Tobacco Guangxi Industrial Co., Ltd., Nanning, China ^{[1
]}

不详 ^{[2
]}

不详 ^{[3
]}

机构：

[1] Technology Center, China Tobacco Guangxi Industrial Co., Ltd., Nanning

[2] College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou

[3] Xinjiang Cigarette Factory, HongyunHonghe Co., Ltd., Urumchi

来源：

Tob. Sci. Technol. | / 8卷 / 25-30期

关键词：

Antioxidative activity; Bud; Oriental tobacco; Polysaccharide; Ultrasound-assisted extraction;

D O I：

10.16135/j.issn1002-0861.20150806

中图分类号：

学科分类号：

摘要：

The technological parameters of ultrasound-assisted extraction of polysaccharides from oriental tobacco buds were optimized with orthogonal test. The crude polysaccharide product was fractionated and purified with a sepharose CL-6B chromatographic column and two polysaccharide fractions (Fr-I and Fr-II) were obtained. The chemical compositions, structural characters and thermal stabilities of Fr-I and Fr-II were analyzed by GC, FT-IR and TGA, and their abilities for scavenging hydroxyl radical and DPPH radical were also determined. The results showed that: 1) The optimized conditions for extracting polysaccharides from oriental tobacco buds were ultrasound power 300 W, ratio of water to raw material 30:1 (mL/g), extracting for 6 min at 70℃. The yield of polysaccharide extracted under above conditions was 2.62%. 2) The molecular weights of Fr-I and Fr-II were 181.97 and 16.27 kDa, respectively. 3) Both Fr-I and Fr-II were heteropolysaccharides, IR spectra revealed that they were of α- and β-configurations, respectively. 4) TGA results indicated that the degradation temperatures (Td) of Fr-I and Fr-II were 195.7 and 180.5℃, respectively. 5) Both Fr-I and Fr-II possessed good abilities for scavenging hydroxyl radical and DPPH radical, and the antioxidative activity of Fr-II was higher than that of Fr-I. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

引用

页码：25 / 30

页数：5

共 12 条

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