Measurement of flow characteristics and oxygen metabolism of plate type bioreactor for in vitro high-density cell culture

被引：0

作者：

Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro ku, Tokyo, 153-8505, Japan ^{[1
]}

机构：

[1] Institute of Industrial Science, University of Tokyo, Tokyo, 153-8505, 4-6-1 Komaba, Meguro ku

来源：

Nihon Kikai Gakkai Ronbunshu, B | 2009年 / 752卷 / 864-870期

关键词：

Bio-fluid mechanics; Biological components and systems; High density culture; Mass transfer; Microfluidics; Oxygen metabolism;

D O I：

10.1299/kikaib.75.752_864

中图分类号：

学科分类号：

摘要：

Engineered tissue of digestive organ requires high cell density around 107̃109 cells/mL. For the production of such a high cell density engineered tissue, namely a bioreactor, microstructured scaffold and the flow system supplying oxygen to the cells, should be designed systematically. For this purpose, the plate type hepatic bioreactor. which was optimally designed to support 108 cells/mL cells, was produced to estimate the flow characteristics and oxygen mebolism. The microstructured scaffold which is composed of aligned square pillars, was designed to allow hepatocytes to attached on the surface of microstructures in cell density of 108 cells/mL. The designed scaffold with microstructures (pillar hight: 109.6 μm, channel: 33.1 μm) was fabricated using PDMS. The flow resistance of fabricated scaffold was measured at flow rate from 0.46 to 2.0 mL/sec. Thereafter, hepatorna cell line llepG 2 were cultured in the scaffold with open culture to compose bioreactor of high cell density around 9.7×107̃1.9×108 cells/mL. Finally, the bioreactor was closed for perfusion cell culture, which simulates in vivo circulation system. Oxygen concentration of inlet and outlet of the bioreactor in perfusion cell culture were measured under the perfusion flow rate from 0.3 to 2.2 mL/min. The result showed that there is a certain flow rate, under which the bioreactor consumes maximum oxygen. Nevertheless maximum cellular oxygen metabolic rate in the bior. eactor is far lower than normal cellular metabolic rate. This trend explains the diffusion resistance on cellular surface and the detachment of cells from the surface of bioreactor at high flow rate.

引用

页码：864 / 870

页数：6

共 10 条

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