DNA extraction and ribosomal DNA-ITS sequence molecule barcode identification from wood tissue of Dalbergia odorifera T. Chen

被引：0

作者：

机构：

[1] School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei

[2] School of Life Science, Anhui Agricultural University, Hefei

[3] Hainan Institute of Forestry, Haikou

来源：

Liu, Sheng-Quan | 1600年 / Editorial Board of Chemistry and Industry of Forest Products卷 / 34期

关键词：

Dalbergia odorifera T. Chen; Dalbergia rimosa Roxb; ITS; Molecular identification; Wood's DNA extraction;

D O I：

10.3969/j.issn.0253-2417.2014.05.017

中图分类号：

学科分类号：

摘要：

The wood of Dalbergia odorifera T. Chen originated from different sites of plantations were sampled as materials in this paper. DNA from the sapwood and heartwood of D. odorifera treated with various temperature was extracted and amplified for the internal transcribed space (ITS) sequences. The effects of various temperature treatments on DNA extraction and amplification for target sequences were analyzed. The rDNA-ITS sequences ofD. odorifera from different habitats and D. rimosa Roxb, a closely related species, were sequenced and their divergences were analyzed. The results showed that there were varying extents of diffusion with the DNA extracts from the heat treatment of 25 ℃ and 65 ℃, and the DNA from the heat treatment of 105 ℃ was degraded and smaller than 250bp. The ITS sequences can be amplified successfully from wood after 25 ℃ heat treatments. The distribution of vriations in ITS2 regions of D. odorifera and D. rimosa was larger than ITS1 regions and there were 6 variable sites in both of ITS regions. D. odorifera and D. rimosa could be distinguished by cluster analysis. Therefore, it could provide theoretical reasons to identify wood of D. odorifera from adulterants by using the rDNA-ITS sequences.

引用

页码：103 / 108

页数：5

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