The effect of pressure on viability, mass fraction of intracellular trehalose and cell shape of different yeast strains was studied. The results show that different yeast strains have different sensitivities and applicabilities; pH of broth has little effect on the sensitivity and applicability. High pressure is propitious to accumulation of intracellular trehalose content, and the higher trehalose content, the stronger ability of adaptation to the change of environment, correspondingly, the more sensitive to high pressure, the more obvious of change of cell shape.