Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation

被引:3
作者
Yang, Qinglian [1 ,2 ]
Wang, Lan [2 ]
Zhou, Wenhao [2 ]
Ge, Jinyang [3 ]
Lu, Xiaotong [2 ]
Liu, Hanyuan [2 ]
Yu, Sen [2 ]
机构
[1] Northeastern Univ, Sch Mat Sci & Engn, Shenyang 110004, Peoples R China
[2] Northwest Inst Nonferrous Met Res, Shaanxi Key Lab Biomed Met Mat, Xian 710016, Peoples R China
[3] Cent South Univ, State Key Lab Powder Met, Changsha 410083, Peoples R China
关键词
Multiscale hierarchical porous titanium; scaffold; Biomechanical adaptation; Osteogenic differentiation; Electrochemical dealloying; BIOMEDICAL APPLICATIONS; MECHANICAL-PROPERTIES; STEM-CELLS; BONE; ALLOY; MICROSTRUCTURE; PERFORMANCE; IMPLANTS; DESIGN;
D O I
10.1016/j.matdes.2024.113534
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
The multiscale hierarchical porous titanium scaffolds significantly improve biomechanical adaptation and biological activity by simulating bone structure, presenting attractive application prospects. To achieve the controllable preparation of multiscale hierarchical porous Ti-3Zr-2Sn-3Mo-25Nb (TLM) scaffolds, this study innovatively proposed the combination of additive manufacturing and electrochemical dealloying. The macroscale pores of TLM were fabricated using selective laser melting (SLM), with a pore size of 640 mu m and a porosity of 70.92 %. Then, a biphasic microstructure was obtained by heat treatment. Finally, the micro- and nano-scale structure was prepared via electrochemical dealloying. The elastic modulus of the multiscale hierarchical porous TLM scaffolds was 1.43 GPa, which closely matched the human bone tissue, indicating excellent biomechanical adaptation. Meanwhile, in vitro experiments further demonstrated that these scaffolds significantly enhanced BMSCs adhesion. The alkaline phosphatase (ALP) activity, calcium deposition, and collagen secretion were up-regulated, while lipid differentiation was down-regulated, indicating the scaffolds exhibited the potential for osseointegration. This research provides a valuable method for the preparation of multiscale hierarchical porous titanium scaffolds and offers innovative insights for the development of customized hardtissue implants.
引用
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页数:10
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