Enhanced recovery of polyhydroxyalkanoates from secondary wastewater sludge of sewage treatment plant: Analysis and process parameters optimization

被引：2

作者：

Kumar V. ^{[1
,2
]}

Srivastava S. ^{[3
]}

Thakur I.S. ^{[1
,3
]}

机构：

[1] Environmental Microbiology and Biotechnology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi

[2] Department of Botany, School of Life Science, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, 495009, Chattisgarh

[3] Amity School of Earth & Environment Science, Amity University Haryana, Manesar, Gurugram, 122413, Haryana

来源：

Bioresource Technology Reports | 2021年 / 15卷

关键词：

3-Hydroxybutyrate; 3-Hydroxyvalerate; !sup]13[!/sup]C NMR spectroscopy; Municipal wastewater sludge; Wastewater Treatment;

D O I：

10.1016/j.biteb.2021.100783

中图分类号：

学科分类号：

摘要：

The present study was aimed to recover and characterize the polyhydroxyalkanoates (PHA) from secondary sludge biomass of sewage treatment plant (STP) extracted by organic solvent under optimized conditions. The maximum recovery yield and purity of PHA were 8.3% and 96%, respectively under optimized conditions of incubation time 72 h, reaction temperature incubation at 75 °C, and sludge solid to chloroform ratio 1:20 (w/v). The maximum PHA concentration was 9.58 g/L, under optimized conditions. Spectroscopic analyses of recovered polymer were revealed the presence of 3-hydroxybutyrate (90%) and 3-hydroxyvalerate (10%) monomers content. PHA was thermally stable at below 260 °C, as confirmed by TGA and DSC analyses. This study concluded that sewage sludge can be a sustainable substitute to recover PHA due to enormously maximum PHA yield, inexpensive feedstock, and subsequently decrease the excess sludge burden generated from STP. © 2021 Elsevier Ltd

引用

共 41 条

[1]

Abdo S.M., Ali G.H., Analysis of polyhydroxybutrate and bioplastic production from microalgae, Bull. Nat. Res. Cent., 43, (2019)

[2]

Albuquerque M.G., Eiroa M., Torres C., Nunes B.R., Reis M.A., Strategies for the development of a side stream process for polyhydroxyalkanoate (PHA) production from sugar cane molasses, J. Biotechnol., 130, pp. 411-421, (2007)

[3]

Standard Methods for Examination of Water and Wastewater, (2017)

[4]

Alsafadi D., Ibrahim M.I., Alamry K.A., Hussein M.A., Mansour A., Utilizing the crop waste of date palm fruit to biosynthesize polyhydroxyalkanoate bioplastics with favorable properties, Sci. Tot. Environ., 737, (2020)

[5]

Akdogan M., Celik E., Purification and characterization of polyhydroxyalkanoate (PHA) from a bacillus megaterium strain using various dehydration techniques, J. Chem. Technol. Biotechnol., 93, 8, pp. 2292-2298, (2018)

[6]

Arumugam A., Senthamizhan S.G., Ponnusami V., Sudalai S., Production and optimization of polyhydroxyalkanoates from non-edible Calophyllum inophyllum oil using Cupriavidus necator, Int. J. Biol. Macromol., 112, pp. 598-607, (2018)

[7]

Bengtsson S., Werker A., Christensson M., Welander T., Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater, Bioresour. Technol., 99, 3, pp. 509-516, (2008)

[8]

Berger E., Ramsay B.A., Ramsay J.A., Chavarie C., Braunegg G., PHB recovery by hypochlorite digestion of non-PHB biomass, Biotechnol. Tech., 3, pp. 227-232, (1989)

[9]

Chaleomrum N., Chookietwattana K., Dararat S., Production of PHA from cassava starch wastewater in sequencing batch reactor treatment system, APCBEE Procedia, 8, pp. 162-172, (2014)

[10]

Choi J.Y., Lee J.K., You Y., Park W.H., Epoxidized polybutadiene as a thermal stabilizer for poly (3-hydroxybutyrate). II. Thermal stabilization of poly (3- hydroxybutyrate) by epoxidized polybutadiene, Fiber. Polym., 4, pp. 195-198, (2003)

← 1 2 3 4 5 →