Determination of tetracyclines and their degradation products in soil using ultra-performance liquid chromatography-tandem mass spectrometry

被引：0

作者：

Chen L. ^{[1
]}

Jia C. ^{[1
]}

Liu B. ^{[1
]}

Zhang Y. ^{[2
]}

机构：

[1] Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing

[2] College of Resource and Environment, Sichuan Agricultural University, Chengdu

来源：

Earth Science Frontiers | 2019年 / 26卷 / 06期

关键词：

Metabolites; Soil; Tetracyclines; Ultra-performance liquid chromatography-tandem mass spectrometry;

D O I：

10.13745/j.esf.sf.2019.10.2

中图分类号：

学科分类号：

摘要：

We developed a method for simultaneous determination of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), doxycycline (DC) and their metabolites in soil by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection. The antibiotics were extracted using mixed solvent of acetonitrile and EDTA-Mcllvaine buffer solution and separated and purified by HLB solid phase extraction column. The purified antibiotics were detected by UPLC-MS/MS with ES(+) ionization and quantified in the multiple reaction monitoring (MRM) mode. Within 0.001-0.5 mg•kg-1 range, we found a good linear relationship (R2>0.99) between the peak areas and concentrations of TCs and their metabolites, with limits of detection (LOD) and limits of quantification (LOQ) reaching 1.085-15.780 and 3.617-42.526 μg•kg-1, respectively. At the three addition levels of 0.05, 0.1 and 0.5 mg•kg-1, the average recovery rates for TCs and their metabolites in soil were 62.1%-87.6%, with relative standard deviation (RSD) of 2.35%-13.42%, except for ATC and EATC (<30%). This method is effective and meets the sensitivity, accuracy and precision demands for detecting residues of TCs and their metabolites in soil. © 2019, Editorial Office of Earth Science Frontiers. All right reserved.

引用

页码：1 / 6

页数：5

共 6 条

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