Time-resolved electromechanical and conductive behavior of nanostructured bilayers tethered to the surface of the electrode with incorporated channel proteins and peptides

被引：0

作者：

Stefanowska, Aleksandra ^{[1
]}

Czapczynski, Michal ^{[1
]}

Koprowski, Piotr ^{[2
]}

Szewczyk, Adam ^{[2
]}

Krysinski, Pawel ^{[1
]}

机构：

[1] Univ Warsaw, Fac Chem, 1 Pasteur Str, PL-02093 Warsaw, Poland

[2] Nencki Inst Expt Biol PAS, Lab Intracellular Ion Channels, 3 Pasteur St, PL-02093 Warsaw, Poland

来源：

BIOELECTROCHEMISTRY | 2025年 / 162卷

关键词：

Tethered bilayer lipid membrane; ROMK2 potassium channel; Gramicidin; Mitochondria; Electromechanical properties; Impedance spectroscopy; Time-resolved chronoamperometry; LIPID-MEMBRANES; BIOMIMETIC MEMBRANES; GRAMICIDIN CHANNELS; IMPEDANCE ANALYSIS; ION-TRANSPORT; MODEL; SPECTROSCOPY; CAPACITANCE; INTERFACE; BINDING;

D O I：

10.1016/j.bioelechem.2024.108848

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of incorporation of mitochondrial inner membrane potassium channel, and channel-forming peptide - Gramicidin on the ion transport and electromechanical properties of model lipid membranes tethered to gold electrode was electrochemically investigated by chronoamperometric and impedance spectroscopy techniques. In the case of the potassium channel the ion transport properties were modulated with channelspecific inhibitor - ATP-Mg2+ complex, whereas in the case of gramicidin peptide - by replacing potassium with sodium ions. The observed two exponential current-time responses of the systems studied were interpreted in terms of ion penetration and electrostriction of tethered lipid bilayer membrane, and conclusions supported with the experiments on alkanethiol self-assembled monolayers of different alkanethiol chain lengths deposited on gold.

引用

页数：9