Daily Consumption of Orange Juice from Citrus sinensis L. Osbeck cv. Cara Cara and cv. Bahia Differently Affects Gut Microbiota Profiling as Unveiled by an Integrated Meta-Omics Approach

被引：0

作者：

Brasili, Elisa ^{[1
,2
]}

Hassimotto, Neuza Mariko Aymoto ^{[1
,2
]}

Del Chierico, Federica ^{[3
]}

Marini, Federico ^{[4
]}

Quagliariello, Andrea ^{[3
]}

Sciubba, Fabio ^{[4
]}

Miccheli, Alfredo ^{[4
]}

Putignani, Lorenza ^{[3
,5
]}

Lajolo, Franco ^{[1
,2
]}

机构：

[1] Department of Food Science and Experimental Nutrition, School of Pharmaceutical Science, University of São Paulo, São Paulo,05508-000, Brazil

[2] Food Research Center (FoRC), CEPID-FAPESP, Research Innovation and Dissemination Centers Sao Paulo Research Foundation, São Paulo,05468-901, Brazil

[3] Unit of Human Microbiome, Children's Hospital, Research Institute Bambino Gesù, Rome,00165, Italy

[4] Department of Chemistry, University of Rome la Sapienza, Rome,00185, Italy

[5] Unit of Parasitology, Children's Hospital and Research Institute Bambino Gesù, Rome,00165, Italy

来源：

Journal of Agricultural and Food Chemistry | 2019年 / 67卷 / 05期

基金：

巴西圣保罗研究基金会;

关键词：

1H NMR spectroscopy - Citrus sinensis L. Osbeck - Crossover design - Gut microbiota - Metabolomics - Operational taxonomic units - Orange juice - Pyrosequencing;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. Cara Cara and cv. Bahia on faecal microbiota and metabolome using an integrated meta-omics approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on 1H NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group. © 2019 American Chemical Society.

引用

页码：1381 / 1391

共 12 条

[11] A comparison of the ultrastructure and composition of fruits’ cuticular wax from the wild-type ‘Newhall’ navel orange (Citrus sinensis [L.] Osbeck cv. Newhall) and its glossy mutant
De-Chun Liu
Qiong Zeng
Qing-Xun Ji
Chuan-Fu Liu
Shan-Bei Liu
Yong Liu
Plant Cell Reports, 2012, 31 : 2239 - 2246
[12] A comparison of the ultrastructure and composition of fruits' cuticular wax from the wild-type 'Newhall' navel orange (Citrus sinensis [L.] Osbeck cv. Newhall) and its glossy mutant
Liu, De-Chun
Zeng, Qiong
Ji, Qing-Xun
Liu, Chuan-Fu
Liu, Shan-Bei
Liu, Yong
PLANT CELL REPORTS, 2012, 31 (12) : 2239 - 2246

← 1 2 →