Prevalence of antibiotic-resistant fecal bacteria in a river impacted by both an antibiotic production plant and urban treated discharges

被引：0
作者：
Sidrach-Cardona, Ricardo [1 ]
Hijosa-Valsero, María [2 ]
Marti, Elisabet [3 ]
Balcázar, José Luis [3 ]
Becares, Eloy [1 ,2 ]
机构：
[1] Instituto de Medio Ambiente, Universidad de León, 24007 León
[2] Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Campus de Vegazana s/n
[3] Catalan Institute for Water Research (ICRA), Parc Científic i Tecnològic de la Universitat de Girona, 17003 Girona
来源：
Science of the Total Environment | 2014年 / 488-489卷 / 01期
关键词：
Antibiotic production plant; Antibiotic resistance; Fecal indicators; River; Sediments; Urban wastewater treatment plant;
D O I：
10.1016/j.scitotenv.2014.04.100
中图分类号：
学科分类号：
摘要：
In this study, the abundance and spatial dynamics of antibiotic-resistant fecal bacteria (Escherichia coli, total coliforms and Enterococcus spp.) were determined in water and sediment samples from a river impacted by both antibiotic production plant (APP) and urban wastewater treatment plant (WWTP) discharges. Agar dilution and disk diffusion methods were also used for antimicrobial susceptibility testing. Two antimicrobial agents, cephalexin (25μg/ml) and amoxicillin (50μg/ml), were evaluated using the agar dilution method for E. coli, total coliforms (TC) and Enterococcus spp., whereas the degree of sensitivity or resistance of E. coli isolates to penicillin (10U), ampicillin (10μg), doxycycline (30μg), tetracycline (30μg), erythromycin (15μg), azithromycin (15μg) and streptomycin (10μg) was performed using the disk diffusion method. Real-time PCR assays were used to determine the prevalence of three antibiotic-resistance genes (ARGs). The agar dilution method showed that most E. coli isolates and TC were resistant to amoxicillin, especially after receiving the APP discharges. Antibiotic resistances to amoxicillin and cephalexin were higher after the APP discharge point than after the WWTP effluent. The disk diffusion method revealed that 100% of bacterial isolates were resistant to penicillin and erythromycin. Multidrug-resistant bacteria were detected and showed a higher proportion at the WWTP discharge point than those in the APP. Highly multidrug-resistant bacteria (resistance to more than 4 antibiotics) were also detected, reaching mean values of 41.6% in water samples and 50.1% in sediments. The relative abundance of the blaTEM, blaCTX-M and blaSHV genes was higher in samples from the treatment plants than in those collected upstream from the discharges, especially for water samples collected at the APP discharge point. These results clearly demonstrate that both the APP and the WWTP contribute to the emergence and spread of antibiotic resistance in the environment. © 2014 Published by Elsevier B.V.
引用
收藏
页码：220 / 227
页数：7
相关论文
共 43 条
[1]  
Alm E.W., Burke J., Spain A., Fecal indicator bacteria are abundant in wet sand at freshwater beaches, Water Res, 37, pp. 3978-3982, (2003)
[2]  
Alonso A., Sanchez P., Martinez J.L., Environmental selection of antibiotic resistance genes, Environ Microbiol, 3, pp. 1-9, (2001)
[3]  
APHA Standard methods for the examination of water and wastewater, (1999)
[4]  
Ash R.J., Mauck B., Morgan M., Antibiotic resistance of Gram-negative bacteria in rivers, United States, Emerg Infect Dis, 8, pp. 713-716, (2002)
[5]  
Baquero F., Martinez J.L., Canton R., Antibiotics and antibiotic resistance in water environments, Curr Opin Biotechnol, 19, pp. 260-265, (2008)
[6]  
Barbosa T.M., Levy S.B., The impact of antibiotic use on resistance development and persistence, Drug Resist Updat, 3, pp. 303-311, (2000)
[7]  
Boon P.I., Cattanach M., Antibiotic resistance of native and faecal bacteria isolated from rivers, reservoirs and sewage treatment facilities in Victoria, south-eastern Australia, Lett Appl Microbiol, 28, pp. 164-168, (1999)
[8]  
Constanzo S.D., Murby J., Bates J., Ecosystem response to antibiotics entering the aquatic environment, Mar Pollut Bull, 51, pp. 218-223, (2005)
[9]  
da Costa P.M., Vaz-Pires P., Bernardo F., Antimicrobial resistance in Enterococcus spp. isolated in inflow, effluent and sludge from municipal sewage water treatment plants, Water Res, 40, pp. 1735-1740, (2006)
[10]  
Davies C.M., Bavor H.J., The fate of stormwater-associated bacteria in constructed wetland and water pollution control pond systems, J Appl Microbiol, 89, pp. 349-360, (2000)
12345