Isolation, Purification and Composition Analysis of Polysaccharides from Spirulina platensis

被引：5

作者：

Fan B. ^{[1
]}

Luo J. ^{[1
]}

Zhang H. ^{[2
]}

Zhang X. ^{[2
]}

Wang L. ^{[1
]}

An Z. ^{[1
]}

Lu H. ^{[1
]}

Zhao X. ^{[1
]}

机构：

[1] College of Food Science and Engineering, Ocean University of China, Qingdao

[2] Qingdao Customs Technology Center, Qingdao

来源：

Shipin Kexue/Food Science | 2022年 / 43卷 / 04期

关键词：

Composition analysis; Rhamnan; Spirulina platensis; Sulfated polysaccharide; Thermal degradation;

D O I：

10.7506/spkx1002-6630-20201104-035

中图分类号：

学科分类号：

摘要：

Objective: The polysaccharides from Spirulina platensis were extracted, purified and thermally degraded. The chemical properties and fine composition of different polysaccharide fractions were analyzed and compared. Methods: Five polysaccharide fractions (P0, P0.2, P0.4, P0.6 and P0.8) and their degradation products were obtained by sequential protease hydrolysis, alcohol precipitation, anion exchange chromatography and thermal degradation. High performance size exclusion chromatography (HPSEC), ion chromatography (IC), high performance liquid chromatography (HPLC) and hydrophilic interaction liquid chromatography-Fourier transform mass spectrometry (HILIC-FTMS) were used to analyze the chemical properties and fine composition of different polysaccharide fractions. Results: The content of sulfated groups in P0, P0.2, P0.4 and P0.6 ranged from 6% to 7%, lower than that in P0.8 (14.46%). The monosaccharide composition analysis showed that P0 was a glucan. P0.2 was a heteropolysaccharide mainly composed of glucose, galactose, rhamnose, fucose and other neutral sugars, while P0.4, P0.6 and P0.8 were mainly composed of rhamnose, glucuronic acid, glucose, xylose and galactose. Through HILIC-FTMS analysis, it was found that the oligosaccharides derived from the thermal degradation of P0.2 were mainly composed of hexose monosaccharide, pentose oligosaccharides, deoxyhexose-pentose oligosaccharides and deoxyhexose-uronic acid oligosaccharides with sulfate groups being in the deoxyhexose, pentose and hexose moieties, and that the composition of the oligosaccharide chain was complicated. However, the composition of P0.4 and P0.6 was very similar, mainly composed of rhamnose-pentose oligosaccharides, rhamnose-glucuronic acid oligosaccharides, pentose disaccharides and trisaccharides with sulfate groups being in the rhamnose and pentose moieties. Conclusion: There are many complex sulfated polysaccharides with different structures in S. platensis, including sulfated glucouronic acid-rhamnan as a unique sulfate polysaccharide. © 2022, China Food Publishing Company. All right reserved.

引用

页码：160 / 166

页数：6

共 11 条

[1] ANANYEV G, CARRIERI D, DISMUKES G C., Optimization of metabolic capacity and flux through environmental cues to maximize hydrogen production by the cyanobacterium "Arthrospira (Spirulina) maxima, Applied & Environmental Microbiology, 74, 19, pp. 6102-6113, (2008)
[2] RAJASEKAR P, PALANISAMY S, ANJALI R, Et al., Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: in vitro antioxidant, antibacterial activity and zebrafish growth and reproductive performance, International Journal of Biological Macromolecules, 141, pp. 809-821, (2019)
[3] DANANJAYA S H S, THU THAO N T, WIJERATHNA H M S M, Et al., In vitro and in vivo anticandidal efficacy of green synthesized gold nanoparticles using Spirulina maxima polysaccharide, Process Biochemistry, 92, pp. 138-148, (2020)
[4] FINAMORE A, PALMERY M, BENSEHAILA S, Et al., Antioxidant, immunomodulating, and microbial-modulating activities of the sustainable and ecofriendly Spirulina, Oxidative Medicine and Cellular Longevity, 2017, pp. 1-14, (2017)
[5] MA H T, XIONG H Y, ZHU X L, Et al., Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice, International Journal of Biological Macromolecules, 133, pp. 1090-1101, (2019)
[6] CHAIKLAHAN R, CHIRASUWAN N, TRIRATANA P, Et al., Effect of extraction temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method, Biotechnology and Bioprocess Engineering, 19, pp. 369-377, (2014)
[7] DE SOUSA ESILVA A, DE MAGALHAES W T, MOREIRA L M, Et al., Microwave-assisted extraction of polysaccharides from Arthrospira (Spirulina) platensis using the concept of green chemistry, Algal Research, 35, pp. 178-184, (2018)
[8] RATANA C, NATTAYAPORN C, VEARA L, Et al., Stepwise extraction of high-value chemicals from Arthrospira (Spirulina) and an economic feasibility study, Biotechnology Reports, 20, (2018)
[9] FU D T, ONEILL R A., Monosaccharide composition analysis of oligosaccharides and glycoproteins by high-performance liquid chromatography, Analytical Biochemistry, 227, 2, pp. 377-384, (1995)
[10] (1999)

← 1 2 →