Evaluation of a new medium for the enumeration of total coliforms and Escherichia coli in Japanese surface waters

被引：0

作者：

Kodaka, H. ^{[1
,2
,3
]}

Mizuochi, S. ^{[1
]}

Saito, M. ^{[2
]}

Matsuoka, H. ^{[2
]}

机构：

[1] Research Institute of Advanced Technology, Nissui Pharmaceutical Co. Ltd., Hokunanmoro, Yuki, Ibaraki, Japan

[2] Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Nakamachi, Koganei, Tokyo, Japan

[3] Research Institute of Advanced Technology, Nissui Pharmaceutical Co. Ltd., 1075-2, Hokunanmoro, Yuki, Ibaraki 307-0036, Japan

来源：

Journal of Applied Microbiology | 2008年 / 104卷 / 04期

关键词：

Aim: A new medium; EC-Blue-10; containing chromogenic and fluorogenic substrates; KNO3 and sodium pyruvate has been developed for the rapid simultaneous detection and enumeration of total coliforms and Escherichia coli in water. Methods and Results: Two evaluations of EC-Blue-10 were carried out. Firstly; EC-Blue-10 was compared with Colilert-MPN for 96 water samples using MPN for total coliforms and E. coli. Secondly; the detection of coliforms and E. coli were compared using 2400 tubes of EC-Blue-10 and Colilert-MPN. The regression coefficients between EC-Blue-10 and Colilert-MPN for total coliforms and E. coli were 0.91 and 0.89; respectively. For the detection results; the Cohen's kappa values between the two media were 0.79 for coliforms and 0.72 for E. coli. Conclusions: EC-Blue-10 is almost same as Colilert-MPN for the detection of coliforms and E. coli in surface waters. Further evaluation for EC-Blue-10 is needed to verify in different geographical areas. Significance and Impact of the Study: EC-Blue-10 is useful method for the rapid and simultaneous detection of total coliforms and E. coli in water sample. © 2007 The Authors;

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摘要：

Journal article (JA)

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页码：1112 / 1118