Molecular mechanisms underlying proliferation and apoptosis in breast cancer MCF-7 cells induced by pentabrominated diphenyl ethers

被引：2
作者：
Department of Environment Hygiene, Chongqing University of Medical Sciences, Chongqing, China [1 ]
机构：
[1] Department of Environment Hygiene, Chongqing University of Medical Sciences, Chongqing
来源：
Toxicol. Environ. Chem. | 2009年 / 4卷 / 665-670期
关键词：
5-BDE; Bax; Bcl-2; MCF-7; cell;
D O I：
10.1080/02772240802343370
中图分类号：
学科分类号：
摘要：
The molecular mechanisms underlying proliferation and apoptosis induced by pentabrominated diphenyl ethers (5-BDE) through regulation of mRNA and protein expression of Bcl-2 and Bax was investigated using breast cancer MCF-7 cells. Human breast cancer MCF-7 cells were grown in DMEM medium, MTT assay was employed to investigate cell proliferation. RT-PCR and immunohistochemistry was performed to determine mRNA and protein expression of Bcl-2 and Bax. Within the range of 1 × 10-8-10-4mol L-1, polybrominated diphenyl ethers (PBDE) stimulated proliferation in MCF-7 cells in a concentration- and time-dependent manner. RT-PCR and immunohistochemistry analyses revealed that treatment with PBDE for 72 h resulted in increased mRNA and protein expression of Bcl-2 and inhibition of Bax. Evidence indicates that 5-BDE exerted estrogenic actions and stimulated proliferation in estrogen responsive breast cancer MCF-7 cells via up-regulation of Bcl-2 mRNA and protein expression. © 2009 Taylor & Francis.
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页码：665 / 670
页数：5
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