Effect of hydrogen peroxide on CO2 laser pretreatment induced drought tolerance in wheat seedlings

被引：0
|
作者：
Qiu Z. [1 ]
Shi J. [1 ]
Zhang M. [1 ]
Yu R. [1 ]
Yue M. [2 ]
机构：
[1] College of Life Science, Henan Normal University
[2] College of Life Science, Northwest University
来源：
Zhongguo Jiguang/Chinese Journal of Lasers | 2010年 / 37卷 / 08期
关键词：
CO[!sub]2[!/sub] laser; Drought stress; Hydrogen peroxide; Laser biology; Medical optics and biotechnology; Physiology experiment;
D O I：
10.3788/CJL20103708.2170
中图分类号：
学科分类号：
摘要：
The objective of this study is to test whether hydrogen peroxide (H2O2) is involved in laser pretreatment induced drought tolerance in wheat seedlings due to its nature as a second messenger in stress responses. Plant is treated with 3 min CO2 laser pretreatment, laser pretreatment in combination with catalase (CAT), ascorbate (AsA) or diphenylene iodonium (DPI) and their effects on the lipid peroxidation, the activities of antioxidant enzymes and the concentration of photosynthesis pigment and seedlings growth and development were compared. The results show that 3 min laser pretreatment can enhance drought tolerance in wheat seedlings by decreasing the concentration of malondialdehyde (MDA) and increasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and the concentration of chlorophyll a, chlorophyll b, carotenoid and plant height, root length and root dry weight. But the promotive effect of laser pretreatment induced drought tolerance in wheat seedling is effectively reversed by the addition of CAT, AsA or DPI. The results suggest that H2O2 is involved in laser pretreatment induced drought tolerance in wheat seedlings and laser induced protective effect is likely related to NADPH oxidase-dependent H2O2 production.
引用
收藏
页码：2170 / 2176
页数：6
相关论文
共 29 条
  • [1] Hernandez A.C., Carballo C.A., Artola A., Et al., Laser irradiation effects on maize seed field performance, Seed Science and Technology, 34, 1, pp. 193-197, (2006)
  • [2] Guo J., Han R., Effects of He-Ne laser on thylakoid membrane characteristic of wheat seedling exposed to enhanced UV-B radiation, Chinese J. Lasers, 36, 3, pp. 758-764, (2009)
  • [3] Qiu Z., Zhu X., Li F., Et al., The optical effect of semiconductor laser on protecting wheat from UV-B radiation damage, Photochemical & Photobiological Sciences, 6, 7, pp. 788-793, (2007)
  • [4] Qiu Z., Zhu X., Li F., Et al., Precaution against ultroviolet-B-induced damage by pre-treating with semiconductor laser in wheat seedlings, Chinese J. Lasers, 34, 8, pp. 1163-1168, (2007)
  • [5] Chen Y.P., Liu Y.J., Wang X.L., Et al., Effect of microwave and He-Ne laser on enzyme activity and biophoton emission of isatis indigotica fort, J. Intagrative Plant Biology, 47, 7, pp. 849-855, (2005)
  • [6] Chen Y.P., Yue M., Wang X.L., Influence of He-Ne laser irradiation on seeds thermodynamic parameters and seedlings growth of isatis indogotica, Plant Science, 168, 3, pp. 601-606, (2005)
  • [7] Han R., Wang X., Yue M., Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA, Chinese Science Bulletin, 47, 6, pp. 435-438, (2002)
  • [8] Qi Z., Yue M., Wang X., Laser pretreatment protects cells of broad bean from UV-B radiation damage, J. Photochemistry and Photobiolsgy B, 59, 1-3, pp. 33-37, (2000)
  • [9] Qi Z., Yue M., Han R., Et al., The damage repair role of He-Ne laser on plants exposed to different intensities of ultraviolet-B irradiation, Photochem. and Photobiol., 75, 6, pp. 680-686, (2002)
  • [10] Qiu Z., Liu X., Tian X., Et al., Effects of CO<sub>2</sub> laser pretreatment on drought stress resistance in wheat, J. Photochemistry and Photobiology B: Biology, 90, 1, pp. 17-25, (2008)
123