Validity of particle-counting method using laser-light scattering for detecting platelet aggregation in diabetic patients

被引：0

作者：

Nakadate, Hiromichi ^{[1
]}

Sekizuka, Eiichi ^{[2
]}

Minamitani, Haruyuki ^{[1
]}

机构：

[1] Graduate School of Science and Technology, Keio University, Kohoku-ku, Yokohama-city, Kanagawa 223-0061

[2] Internal Medicine, National Hospital Organization Saitama Hospital, Wako-city, Saitama 351-0102

来源：

IEEJ Transactions on Electronics, Information and Systems | 2007年 / 127卷 / 10期

关键词：

Diabetes; Light scattering; Particle counting method; Platelet aggregation;

D O I：

10.1541/ieejeiss.127.1515

中图分类号：

学科分类号：

摘要：

We aimed to study the validity of a new analytical approach that reflected the phase from platelet activation to the formation of small platelet aggregates. We hoped that this new approach would enable us to use the particle-counting method with laser-light scattering to measure platelet aggregation in healthy controls and in diabetic patients without complications. We measured agonist-induced platelet aggregation for 10 min. Agonist was added to the platelet-rich plasma 1 min after measurement started. We compared the total scattered light intensity from small aggregates over a 10-min period (established analytical approach) and that over a 2-min period from 1 to 3 min after measurement started (new analytical approach). Consequently platelet aggregation in diabetics with HbAlC ≥ 6.5% was significantly greater than in healthy controls by both analytical approaches. However, platelet aggregation in diabetics with HbAlC < 6.5%, i.e. patients in the early stages of diabetes, was significantly greater than in healthy controls only by the new analytical approach, not by the established analytical approach. These results suggest that platelet aggregation as detected by the particle-counting method using laser-light scattering could be applied in clinical examinations by our new analytical approach.

引用

页码：1515 / 1521+4

共 22 条

[1] Born G.V., Aggregation of blood platelets by adenosine diphosphate and its reversal, Nature, 194, pp. 927-929, (1962)
[2] Born G.V., Observations on the change in shape of blood platelets brought about by adenosine diphosphate, J Physiol., 209, 2, pp. 487-511, (1970)
[3] Born G.V., Hume M., Effects of the numbers and sizes of platelet aggregates on the optical density of plasma, Nature, 215, 5105, (1967)
[4] Kitek A., Breddin K., Optical density variations and microscopic observations in the evaluation of platelet shape change and microaggregate formation, Thromb Haemost, 44, 3, pp. 154-158, (1980)
[5] Gear A.R., Lambrecht J.K., Reduction in single platelets during primary and secondary aggregation, Thromb Haemost., 45, 3, (1981)
[6] Frojmovic M.M., Milton J.G., Duchastel A., Microscopic measurements of platelet aggregation reveal a low ADP-dependent process distinct from turbidometrically measured aggregation, J Lab Clin Med., 101, 6, pp. 964-976, (1983)
[7] Thompson N.T., Scrutton M.C., Walks R.B., Particle volume changes associated with light transmittance changes in the platelet aggregometer: Dependence upon aggregating agent and effectiveness of stimulus, Thromb Res., 41, 5, pp. 615-626, (1986)
[8] Ozaki Y., Satoh K., Yatomi Y., Yamamoto T., Shirasawa Y., Kume S., Detection of platelet aggregates with a particle counting method using light scattering, Anal Biochem., 218, 2, pp. 284-294, (1994)
[9] Yamamoto T., Egawa Y., Shirasawa Y., Ozaki Y., Sato K., Yatomi Y., Kume H., A laser light scattering in situ system for counting aggregates in blood platelet aggregation, Meas. Sci. Technol., 6, pp. 174-180, (1995)
[10] Diminno G., Silver M.J., Cerbone A.M., Riccardi G., Rivellese A., Mancini M., Platelet fibrinogen binding in diabetes mellitus. Differences between binding to platelets from nonretinopathic and retinopathic diabetic patients, Diabetes, 35, 2, pp. 182-185, (1986)

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