The contents of the potassium, calcium, sodium, magnesium, copper, zinc, iron, manganese, strontium and nickel in Dioscorea opposita thumb were determined by use of flame atomic absorption spectrophotometry. The relative standard deviation (RSD) of the first eight contents is 0.43%, 1.10%, 4. 41%, 0.68%, 1.44%, 1.88%, 1.29% and 0.03% respectively, and the percentage recovery is 90.0%-111.0%. The method is convenient and accurate. It can be used to determine the trace elements simultaneously in Dioscorea opposita thumb.