Optimization of HS-SPME-GC-MS for the Characterization of Key Volatile Compounds in Lentinus edodes at Different Maturity Stages

被引:0
作者
优化HS-SPME-GC-MS方法表征香菇不同成熟阶段的关键挥发性化合物
机构
[1] Food Science College, Shenyang Agricultural University, Shenyang
来源
Xin, Guang (xinguang@syau.edu.cn) | 2025年 / 46卷 / 01期
关键词
aroma; gas chromatography-mass spectrometry; headspace solid-phase microextraction; Lentinus edodes; maturity stage; partial least squares discriminant analysis; volatile compounds;
D O I
10.7506/spkx1002-6630-20231227-240
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The volatile organic compounds (VOCs) in Lentinus edodes at different maturity stages were identified by optimized headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (GC-MS) and analyzed by odor activity value (OAV) and partial least squares discriminant analysis (PLS-DA). The results showed that the optimal extraction conditions were as follows: 1.0 g of sample was extracted at 50 ℃ for 25 min and then the analytes adsorbed on the fiber were desorbed in the GC-MS injection port for 3 min. A total of 71 VOCs were identified in L. edodes, and there were significant differences in the types and contents of volatile compounds at different maturity stages (P < 0.05). Out of these, 18 VOCs that could be used as biomarkers to distinguish L. edodes at different maturation stages were selected by PLS-DA and variable importance in projection (VIP). Besides, 16 volatile compounds with OAV ≥ 1 were identified. Among these compounds, 1-octen-3-ol, 3-octanol, 1-octen-3-one, 3-octanone, benzeneacetaldehyde, dimethyl disulphide, dimethyl trisulphide, 2,3,5-trithiohexane, and 1,2,4-trithiohexane showed VIP greater than 1, thus making them the most significant differential volatile compounds among different maturity stages of L. edodes. This study provides a theoretical foundation for understanding the mechanism of aroma formation during the maturation process of L. edodes. © 2025 Chinese Chamber of Commerce. All rights reserved.
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页码:74 / 82
页数:8
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