Poly(GMA-co-VFc)/Fe3O4/cholesterol oxidase bionanocomposite based electrodes for amperometric cholesterol biosensor

被引：0
作者：
机构：
[1] Department of Geneticcs and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul
[2] Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul
来源：
enel, Mehmet | 1600年 / American Scientific Publishers卷 / 12期
关键词：
Amperometric biosensor; Bionanocomposite; Cholesterol; Mediator;
D O I：
10.1166/sl.2014.3338
中图分类号：
学科分类号：
摘要：
A highly sensitive cholesterol biosensor was fabricated using poly(GMA-co-VFc)/MNP and cholesterol oxidase. The enzyme was immobilized on to glassy carbon electrode surface using poly(GMAco- VFc) redox polymer containing super paramagnetic iron oxide nanoparticles (MNPs), which greatly enhanced electron transport between electrode and enzyme, giving a biosensor very good sensitivity, 47 μA mM-1, linear range of 0.01-0.12 μM, a detection limit of 0.005 μM and incredible response time of 1 s. The biosensor retained 70% of its original response after 18 days, when stored in 0.1 M PBS (pH 7.0) at 4 °C, and it losses 18% of its initial activity in 20 continues assays. Copyright © 2014 American Scientific Publishers All rights reserved.
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页码：1507 / 1512
页数：5
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