In vitro selection of dye-fluorescence-enhancing peptide aptamer by cDNA display

被引:1
作者
Kubo, Takashi [1 ]
Koike, Tomoyuki [1 ]
Ouchi, Tomoki [1 ]
Khaliq, Nayab [1 ]
Sasaki, Eita [2 ]
Kuroda, Kouichi [3 ]
Ueda, Mitsuyoshi [4 ]
Hanaoka, Kenjiro [2 ]
Nemoto, Naoto [1 ]
机构
[1] Saitama Univ, Grad Sch Sci & Engn, 255 Shimo Okubo,Sakura Ku, Saitama, Saitama 3388570, Japan
[2] Keio Univ, Grad Sch Pharmaceut Sci, 1-5-30 Shibakoen,Minato Ku, Tokyo 1058512, Japan
[3] Kyoto Inst Technol, Grad Sch Sci & Technol, Matsugasaki,Sakyo Ku, Kyoto, 6068585, Japan
[4] Kyoto Univ, Grad Sch Agr, Kitashirakawa Oiwake Cho,Sakyo Ku, Kyoto 6068502, Japan
关键词
cDNA display; Peptide aptamer; Non-fluorescent dye; Cell imaging; BINDING PEPTIDE; PUROMYCIN; PROTEINS; ACID;
D O I
10.1016/j.ab.2024.115722
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Although Green Fluorescent Protein (GFP) is useful and most widely used, steric hindrance due to its size and the time required for chromophore formation are complications. However, it is difficult to form chromophores with peptides to reduce the molecular weight. Therefore, we focused on peptides that can become fluorescent by binding to dyes. In this study, a novel dye-fluorescence-enhancing peptide aptamer was selected by the cDNA display method, which was confirmed by the yeast surface display method. This peptide aptamer binds to the non-fluorescent dye QSY (R) 9 and enhances its fluorescence by preventing rotation of its benzene sulfone group. The method described in this paper should enable the development of new cell imaging methods using nonfluorescent dyes and peptides.
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页数:7
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