Hofmeister ion effects induced by different acidifiers and alkalizers improve the techno-functional properties of complex rapeseed protein during pH-driven self-assembly

pH-driven method is an effective strategy to prepare complex protein. This study provides guidance on how to select acidifiers and alkalizers from view of Hofmeister ion effects. Cations and anions regulated the molecular structure (particle size, surface charge, protein folding/unfolding, structural orderliness) of complex rapeseed proteins (CRPs) mainly via electrostatic and hydrogen bond. No evident changes were found in the molecular weight distribution, but their distribution on oil/air-water interface varied greatly. Various techno-functional properties of CRPs were synergistically improved: Citrate3- and Na+ increased the emulsifying activity index of CRPs from 80 to 102.21 m2/g; Citrate3-, K+ and Na+ made the foaming stability of CRPs close to 80 % after 60 min of storage. Moreover, the oil/water-holding and gel properties of CRPs were regulated effectively. These findings demonstrate the key role of Hofmeister ion effects in improving CRPs properties, contributing to develop, select, and apply novel acidifiers and alkalizers during pH-driven treatment.