Protective effect of mogroside extract on ethanol-induced l-02 hepatocytes damage

被引：0
|
作者：
Zhu, Huiling [1 ]
Liu, Hesheng [2 ]
Qi, Xiangyang [2 ]
Chen, Qiuping [2 ]
Zhang, Yali [3 ]
机构：
[1] College of Food Science and Technology, Huazhong Agricultural University, Wuhan
[2] College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, Zhejiang
[3] College of Food Science and Technology, Shanghai Ocean University, Shanghai
来源：
Journal of Chinese Institute of Food Science and Technology | 2015年 / 15卷 / 01期
关键词：
Ethanol; Hepatocytes damage; L-02; hepatocytes; Mogroside extract; Oxidative stress;
D O I：
10.16429/j.1009-7848.2015.01.003
中图分类号：
学科分类号：
摘要：
This study aimed at investigating the protective effect of mogroside extract (MG) against ethanol-induced L-02 hepatocytes injury in vitro. The results showed that no obvious cytotoxicity was observed on cultured L-02 hepatocytes at a dose 10-500 μmol/L. Pretreatment with 100 or 200 μmol/L MG has restored the shape and growth of L-02 near normal group (P<0.05). Furthermore, pretreatment with 50-200 μmol/L MG remarkably decreased the activities of ALT and LDH on L-02 cell culture supernatant and intracellular MDA level (P<0.05) together with significant increase in intracellular SOD activity and GSH level (P<0.05). These findings indicated that pretreatment with MG maintained L-02 membrane integrity, enhanced cellular antioxidant ability and prevented lipid peroxidation. That meaned that MG suppressed ethanol-induced hepatocytes injury in vitro. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.
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页码：13 / 18
页数：5
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