Bacillus cereus cell and spore properties as influenced by the micro-structure of the medium

被引：0

作者：

Stecchini, M.L. ^{[1
,2
]}

Spaziani, M. ^{[2
]}

Torre, M. Del ^{[2
]}

Pacor, S. ^{[3
]}

机构：

[1] Department of Food Science, University of Udine, Via Sondrio 2, 33100 Udine, Italy

[2] Department of Food Science, University of Udine, Udine, Italy

[3] Department of Biomedical Science, University of Trieste, Trieste, Italy

来源：

Journal of Applied Microbiology | 2009年 / 106卷 / 06期

关键词：

Aim: To investigate the effect of different growth conditions on Bacillus cereus cell and spore properties. Methods and Results: Bacillus cereus was grown on agar plates with different surface water conditions (wet and dry) or viscosity. Cell populations displayed different types of behaviour; and heterogeneity was manifested in cell motility and dimension. Spore populations were heterogeneous regarding their properties; namely size and thermal resistance. The smallest spores were produced from flagellated cells; which also displayed jet-motility; growing on the wettest agar. Cytometric analysis also revealed within the smallest spores a sub-population labelled by propidium iodide (PI); indicating that spore populations were partly damaged. Nonmotile cells grown on diffusion-limiting media were elongated and produced the least thermal-resistant spores. Conclusions: The micro-structural properties of the media were found to influence cell and spore properties. Abundant surface water enabled flagellar motility and resulted in a heterogeneous cell and spore population; the latter including small and damaged spores. High viscosity gave rise to filamentous cells and more heat-sensitive spores. Significance and Impact of the Study: This study provides useful information on conditions resulting in heterogeneous populations of damaged and heat-sensitive spores. © 2009 The Society for Applied Microbiology;

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Journal article (JA)

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页码：1838 / 1848