Development of a real-time PCR for rapid detection of Skeletonema costatum

被引：0

作者：

He, Shanying

Cheng, Gang

Gou, Wanli

Li, Rongxiu

Mi, Tiezhu

Yu, Zhigang

机构：

[1] Ocean University of China, Qingdao 266003, China

[2] Qingdao Eentry-Exit Inspection and Quarantine Bureau, Qingdao 266101, China

[3] College of Life Science and Technology, Shanghai Jiaotong University, Shanghai 200030, China

来源：

Gaojishu Tongxin/Chinese High Technology Letters | 2006年 / 16卷 / 03期

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摘要：

Sequences of the rDNA and ITS deduced from Skeletonema costatum were used for designing species-specific primers, and a real-time fluorescent quantitative polymerase chain reaction (RFQ-PCR) method was developed for quantitative detection of S. costatum. Seven sets of primers and probes were designed, and were applied to specificity check with PCR and dot-blot analysis. Probe 6 and primer 6 (F/R) were selected to develop a RFQ-PCR method for S. costatum. A calibration curve was constructed with Cycle Threshold value against visual counted cell number. The value of the curve was tested with other S. costatum samples, which were assayed with both RFQ-PCR method and visual count under microscope.

引用

页码：313 / 318