Site-saturated mutagenesis of histidine 234 of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase demonstrates dual functions in cyclization and rearranaement reactions

被引:0
|
作者
Wu, Tung-Kung [1 ]
Liu, Yuan-Ting [1 ]
Chang, Cheng-Hsiang [1 ]
Yu, Mei-Ting [1 ]
Wang, Hisng-Ju [1 ]
机构
[1] Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin-Chu, Taiwan
来源
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Journal article (JA)
引用
收藏
页码:6414 / 6419
相关论文
共 13 条
  • [1] Site-saturated mutagenesis of histidine 234 of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase demonstrates dual functions in cyclization and rearrangement reactions
    Wu, Tung-Kung
    Liu, Yuan-Ting
    Chang, Cheng-Hsiang
    Yu, Mei-Ting
    Wang, Hisng-Ju
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (19) : 6414 - 6419
  • [2] Histidine residue at position 234 of oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae simultaneously influences cyclization, rearrangement, and deprotonation reactions
    Wu, TK
    Liu, YT
    Chang, CH
    CHEMBIOCHEM, 2005, 6 (07) : 1177 - +
  • [3] ORGN 608-Enzymatic formation of multiple triterpenes by mutation of histidine 234 of the oxidosqualene-lanosterol cyclase from saccharomyces cerevisiae
    Liu, Yuan-Ting
    Wu, Tung-Kung
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 232
  • [4] Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions
    Wu, TK
    Yu, MT
    Liu, YT
    Chang, CH
    Wang, HJ
    Diau, EWG
    ORGANIC LETTERS, 2006, 8 (07) : 1319 - 1322
  • [5] ORGN 609-Site-saturated mutagenesis of phenylalanine 445 of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase results in production of isomalabaricane triterpenoid and parkeol
    Wu, Tung-Kung
    Liu, Yuan-Ting
    Chiu, Feng-Hsuan
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 232
  • [6] Phenylalanine 445 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences C-ring cyclization and deprotonation reactions
    Wu, Tung-Kung
    Liu, Yuan-Ting
    Chiu, Feng-Hsuan
    Chang, Cheng-Hsiang
    ORGANIC LETTERS, 2006, 8 (21) : 4691 - 4694
  • [7] Alteration of the Substrate's Prefolded Conformation and Cyclization Stereochemistry of Oxidosqualene-Lanosterol Cyclase of Saccharomyces cerevisiae by Substitution at Phenylalanine 699
    Wu, Tung-Kung
    Chang, Cheng-Hsiang
    Wen, Hao-Yu
    Liu, Yuan-Ting
    Li, Wen-Hsuan
    Wang, Tsai-Ting
    Shie, Wen-Shiang
    ORGANIC LETTERS, 2010, 12 (03) : 500 - 503
  • [8] The cysteine 703 to isoleucine or histidine mutation of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae generates an iridal-type triterpenoid
    Chang, Cheng-Hsiang
    Chen, Yi-Chi
    Tseng, Sheng-Wei
    Liu, Yuan-Ting
    Wen, Hao-Yu
    Li, Wen-Hsuan
    Huang, Chiao-Ying
    Ko, Cheng-Yu
    Wang, Tsai-Ting
    Wu, Tung-Kung
    BIOCHIMIE, 2012, 94 (11) : 2376 - 2381
  • [9] Functional analysis of alanine-scanning and site-directed mutations in the oxidosqualene-lanosterol cyclase gene of Saccharomyces cerevisiae
    Wu, TK
    Wang, HJ
    Chiu, FH
    FASEB JOURNAL, 2001, 15 (04): : A186 - A186
  • [10] ORGN 610-Identification of a putative active site residue within oxidosqualene-lanosterol cyclase from saccharomyces cerevisiae that influences B/C ring cyclization
    Li, Wen-Hsuan
    Wu, Tung-Kung
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 232