A novel genotypic test for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates by a multiplex probe array

被引：0
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作者：
Zhang, S.-L. [1 ,2 ]
Shen, J.-G. [3 ]
Xu, P.-H. [4 ]
Li, D.-X. [1 ]
Sun, Z.-Q. [5 ]
Li, L. [1 ]
Yang, Z.-R. [1 ]
Sun, Q. [1 ,6 ]
机构：
[1] College of Life Sciences, Key Laboratory of Bio-resource and Bio-control, Sichuan University, Chengdu, Sichuan, China
[2] Research Center for Tuberculosis, Henan Chest Hospital, Zhengzhou, Henan, China
[3] College of Basic Education, Nanyang Institute of Technology, Nanyang, Henan, China
[4] Research Center for Bioengineering of Henan, Zhengzhou, Henan, China
[5] Department of Microbiology, Henan Chest Hospital, Zhengzhou, Henan, China
[6] College of Life Sciences, Sichuan University, 29# Wangjianglu St, Chengdu, Sichuan 610064, China
来源：
Journal of Applied Microbiology | 2007年 / 103卷 / 04期
关键词：
Aims: To develop and evaluate a novel genotypic test for rapid detection of rifampicin and isoniazid resistance of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates by a multiplex probe array. Methods and Results: A multiplex probe array was designed for genotypic test to simultaneously screen the mutations of rpoB; katG; inhA and ahpC genes; associated with rifampin and isoniazid resistance in M. tuberculosis; with a probe detecting one of the recently confirmed genetic markers of isoniazid resistance ahpC-6 and -9 locus added. By using the genotypic test developed; 52 MDR isolates were identified; among which 46 isolates had mutations in rpoB (88.5%) and 45 at codon 315 of katG; regulatory region of inhA and oxyR-ahpC intergenic region (86.5%); whereas all 35 susceptible isolates identified showed a wild-type hybridization pattern. The sensitivity and specificity were 88.5% and 100% for rifampicin resistance; and 86.5% and 100% for isoniazid resistance; respectively. Conclusion: A rapid and simultaneous detection of rifampicin and isoniazid resistance caused by the mutations of rpoB; inhA and ahpC genes in M. tuberculosis isolates could be achieved by a multiplex probe array developed. Significance and Impact of the Study: This genotypic test protocol has the potential to be developed on clinical application for the rapid detection of drug resistant M. tuberculosis isolates before an efficient chemotherapy is initiated. © 2007 Sichuan University;
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页码：1262 / 1271
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