Fabrication of an electrogenerated chemiluminescence biosensor based on CdSe quantum dots for the determination of Acetylcholine

被引：0

作者：

Park, Jun-Su ^{[1
]}

Kang, Yun Ok ^{[1
]}

Lee, So-Ra ^{[1
]}

Bae, Hyunmin ^{[2
]}

Choi, Sunwoong ^{[2
]}

Choi, Seong-Ho ^{[1
]}

机构：

[1] Department of Chemistry, Hannam University, Daejeon

[2] Department of Polymer Science and Engineering, Hannam University, Daejeon

来源：

Sensor Letters | 2015年 / 13卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Biosensor; Cadmium selenide; Electrogenerated chemiluminescence; L-cystein; Quantum dots;

D O I：

10.1166/sl.2015.3415

中图分类号：

学科分类号：

摘要：

A novel form of cadmium selenide quantum dots (CdSe QDs) was constructed and used as an electrogenerated chemiluminescence (ECL) and electrochemical probe for the detection of biomaterials, DNA, and cancer cells. A simple and rapid method was demonstrated for an ECL biosensor based on CdSe QDs. In order to improve the intensity of the ECL signal, an ECL biosensor based on CdSe QDs was fabricated by the immobilization of CdSe QDs and acetylcholine after modifying the surface of a glassy carbon electrode (GCE) with 4-aminothiophenol diazonium salts by the electrochemical method. For the detection of acetylcholine concentration, Acetylcholinesterase (AChE) was immobilized onto the fabricated ECL biosensor. The fabricated ECL biosensor based on CdSe QDs was characterized using a scanning electron microscope (SEM), UV-vis spectrophotometry, transmission electron microscopy (TEM), fluorescence spectrometer (PL), and cyclic voltammetry (CV). The fabricated ECL biosensor based on CdSe QDs is suitable for the detection of acetylcholine concentrations in real human blood samples. Copyright © 2015 American Scientific Publishers

引用

页码：85 / 91

页数：6

共 17 条

[1]

Bertoncello P., Forster R.J., Biosensor and Bioelectronics, 24, (2009)

[2]

Kurita R., Arai K., Nakamoto K., Kato D., Niwa O., Anal. Chem., 82, (2010)

[3]

Liu S., Ju H.X., Anal. Chem., 80, (2008)

[4]

Deng S.Y., Lei J.P., Huang Y., Cheng Y., Ju H.S., Anal. Chem., 85, (2013)

[5]

Hu X.-W., Mao C.-J., Song J.-M., Niu H.-L., Zhang S.-Y., Huang H.-P., Biosens. Bioelectron., 41, (2013)

[6]

Wang T., Zhang S., Mao C., Song J., Niu H., Jin B., Tian Y., Biosens. Bioelectron., 31, (2012)

[7]

Rivera V.R., Gamez F.J., Keener W.K., White J.A., Poli M.A., Anal. Biochem., 353, (2006)

[8]

Cui H., Xu Y., Zhang Z.-F., Anal. Chem., 76, (2004)

[9]

Miao W.J., Choi J.P., Bard A.J., Journal of American Chemical Society, 124, (2002)

[10]

Jung D.J., Piao M.-H., Oh S.-H., Woo J.-C., Choi S.-H., J. Nanosci. Nanotechnol., 10, (2010)

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