Synthesis pathway relationship between starch and fat in Chlorella sp. under nitrogen starvation

被引：0

作者：

Wang, Yajie ^{[1
,2
]}

Zhu, Shunni ^{[1
]}

Wang, Zhongming ^{[1
]}

Yuan, Zhenhong ^{[1
]}

机构：

[1] Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, Guangdong

[2] University of Chinese Academic of Sciences, Beijing

来源：

Huagong Xuebao/CIESC Journal | 2015年 / 66卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Biofuel; Biotechnology; Chlorella sp; Fatty acids; Inhibition; Metabolism; Nitrogen starvation; Starch;

D O I：

10.11949/j.issn.0438-1157.20141760

中图分类号：

学科分类号：

摘要：

Triacylglycerol (TAG) and starch, as the two main energy storage products in green microalgae, especially the Chlorella sp., share the same synthesis precursor in its metabolisim system. This research aimed at the key enzymes in inhibiting starch and TAG synthesis, using Chlorella sp. as the object under nitrogen starvation. To investigate the relationship between the two metabolism pathways, comparison of TAG and starch accumulation was conducted. The starch synthesis way was significantly inhibited when 5 mmol·L-1 Pi was added, resulting in reduced starch content and increased fatty acids concentration. While, fatty acids content per cell unit was not obviously influenced. In addition, 40 μmol·L-1 of sethoxydim also showed starch inhibition after it had been introduced for 6 days. In this situation, starch concentration was reduced but unit cell starch content was not significantly changed. ©All Right Reserved

引用

页码：2189 / 2195

页数：6

共 26 条

[1]

Huang W., Zhu S., Wang Z., Xu J., Yuan Z., Synthesis patterns of carbohydrate and fatty acid under nitrogen stravation in the microalgae Chlorella zofingiensis, Acta Energiae Solaris Sinica, 35, 12, pp. 2559-2564, (2014)

[2]

Zhu S., Huang W., Xu J., Wang Z., Yuan Z., Metabolic changes of starch and lipid triggered by nitrogen starvation in the microalga Chlorella zofingiensis, Bioresource Technology, 152, pp. 292-298, (2014)

[3]

Cakmak T., Angun P., Demiray Y.E., Ozkan A.D., Elibol Z., Tekinay T., Differential effects of nitrogen and sulfur deprivation on growth and biodiesel feedstock production of Chlamydomonas reinhardtii, Biotechnology and Bioengineering, 109, 8, pp. 1947-1957, (2012)

[4]

Ball S.G., Morell M.K., From bacterial glycogen to starch: Understanding the biogenesis of the plant starch granule, Annual Review of Plant Biology, 54, pp. 207-233, (2003)

[5]

Radakovits R., Jinkerson R.E., Darzins A., Posewitz M.C., Genetic engineering of algae for enhanced biofuel production, Eukaryot Cell, 9, 4, pp. 486-501, (2010)

[6]

Greenwell H.C., Laurens L.M.L., Shields R.J., Lovitt R.W., Flynn K.J., Placing microalgae on the biofuels priority list: a review of the technological challenges, Journal of the Royal Society Interface, 7, 46, pp. 703-726, (2010)

[7]

Hu Q., Sommerfeld M., Jarvis E., Ghirardi M., Posewitz M., Seibert M., Darzins A., Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances, Plant J, 54, 4, pp. 621-639, (2008)

[8]

Ruuska S.A., Girke T., Benning C., Ohlrogge J., Contrapuntal networks of gene expression during Arabidopsis seed filling, Plant Cell, 14, 6, pp. 1191-1206, (2002)

[9]

Vigeolas H., Mohlmann T., Martini N., Neuhaus H.E., Geigenberger P., Embryo specific reduction of ADP-Glc pyrophosphorylase leads to an inhibition of starch synthesis and a delay in oil accumulation in developing seeds of oilseed rape, Plant Physiol, 136, 1, pp. 2676-2686, (2004)

[10]

Wang Z.T., Ullrich N., Joo S., Waffenschmide S., Goodenough U., Algal lipid bodies: stress induction, purification, and biochemical char-acterization in wild-type and starchless Chlamydomonas reinhardtii, Eukaryotic Cell, 8, 12, pp. 1856-1868, (2009)

← 1 2 3 →