Ethanol production from starch by co-immobilized amyloglucosidase - zymomonas mobilis cells in a continuously-stirred bioreactor

被引：0
作者：
Altuntaş, Evrim Güneş [1 ]
Özçelik, Filiz [2 ]
机构：
[1] Ankara University, Biotechnology Institute, Ankara
[2] Ankara University, Engineering Faculty, Food Engineering Department, Ankara
来源：
Biotechnology and Biotechnological Equipment | 2013年 / 27卷 / 01期
关键词：
Amyloglucosidase (AMG); Co-immobilization; Continuously-stirred bioreactor; Simultaneous saccharification and ethanol fermentation; Zymomonas mobilis;
D O I：
10.5504/bbeq.2012.0111
中图分类号：
学科分类号：
摘要：
Continuous ethanol production from starch was studied in a continuously-stirred bioreactor using co-immobilized amyloglucosidase (AMG) and Zymomonas mobilis cells. In this process, saccharification of starch and fermentation to ethanol were carried out in a single reactor. The experiments were carried out at three sets of conditions for which temperature and centrifuged cell weight were 35 °C and 20.3 g; 30 °C and 19.0 g; 35 °C and 29.2 g, respectively. The maximum volumetric ethanol productivity (7.6 g·L-1·h-1) was achieved with 0.45 g·g-1 yield at the 0.20 h-1 dilution rate at 35 °C, with the initial centrifuged cell concentration of 29.2 g. The maximum ethanol concentration (43.5 g·L-1) was obtained at the 0.15 h-1 dilution rate with a 96 % conversion of substrate. When experimental conditions in the reactor were compared to each other, the initial concentration of immobilized cells was found to have a significant positive influence on the kinetic parameters of ethanol fermentation.
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页码：3506 / 3512
页数：6
相关论文
共 27 条
  • [1] Antoni D., Zverlov V.V., Schwarz W.H., Appl. Microbiol. Biotechnol, 77, pp. 23-35, (2007)
  • [2] Bai F.W., Anderson W.A., Moo-Young M., Biotechnol. Adv, 26, pp. 89-105, (2008)
  • [3] Bai F.W., Chen L.J., Zhang Z., Anderson W.A., Moo-Young M., J. Biotechnol, 110, pp. 287-293, (2004)
  • [4] Bandaru V.V.R., Somalanka S.R., Mendu D.R., Madicherla N.R., Chityala A., Enzyme Microb. Tech, 38, pp. 209-214, (2006)
  • [5] Dien B.S., Cotta M.A., Jeffries T.W., Appl. Microbiol. Biotechnol, 63, pp. 258-266, (2003)
  • [6] Forouchi E., Gunn D.J., Biotechnol Bioeng, 25, pp. 1905-1911, (1983)
  • [7] Georgieva T.I., Ahring B.K., Appl. Microbiol. Biotechnol, 77, pp. 61-68, (2007)
  • [8] Hellendoorn L., Ottengraf S.P., van den Heuvel J.C., Pennings J.A., dos Santos V.A., Wijffels R.H., Biotechnol. Bioeng, 6, pp. 694-704, (1999)
  • [9] Kadar Z., Szengyel K., Reczey K., Ind. Crop Prod, 20, pp. 103-110, (2004)
  • [10] Kim C.H., Rhee S.K., Process Biochem, 28, pp. 331-339, (1993)
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