Chemopreventive role of Indigofera aspalathoides against 20-methylcholanthrene-induced carcinogenesis in mouse

被引：12
作者：
Haldar P.K. [1 ]
Bhattacharya S. [2 ]
Bala A. [1 ]
Kar B. [1 ]
Mazumder U.K. [1 ]
Dewanjee S. [1 ]
机构：
[1] Department of Pharmaceutical Technology, Jadavpur University, Kolkata 32, West Bengal
[2] Bengal School of Technology, West Bengal, Sugandha
来源：
Toxicological and Environmental Chemistry | 2010年 / 92卷 / 09期
关键词：
20-methylcholanthrene; Antioxidant; Chemoprevention; Sarcoma;
D O I：
10.1080/02772241003783703
中图分类号：
学科分类号：
摘要：
This investigation was undertaken to evaluate the chemopreventive effect of hydroalcoholic extract of Indigofera aspalathoides (HAIA) against 20-methylcholanthrene (20-MC)-induced carcinogenesis in mice. Tumor was induced by single subcutaneous administration of 20-MC (200 μg per mouse) in Swiss albino mice. After 24 h of 20-MC administration, HAIA was administered at the doses of 250 and 500 mg kg-1 body weight orally for 90 consecutive days. Mice of all groups were observed for 15 weeks to record tumor incidence (fibrosarcoma) and survival time. After 15 weeks the mice were sacrificed for the estimation of hematological profiles like hemoglobin (Hb), white blood cell (WBC), red blood cell (RBC), and liver biochemical parameters, namely lipid peroxidation, reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT). HAIA treatment markedly reduced tumor incidence and prolonged the life span of sarcoma-bearing mice as compared to 20-MC control mice. Hematological profiles were significantly (p < 0.001) restored to normal levels in HAIA-treated mice as compared to 20-MC control mice. HAIA treatment significantly (p < 0.001) modulated the aforesaid liver biochemical parameters as compared to 20-MC control. The results concluded that I. aspalathoides demonstrated a remarkable chemopreventive effect in chemical-induced carcinogenesis in mouse. The potential chemopreventive action may be due to its antioxidant and detoxifying properties. © 2010 Taylor & Francis.
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页码：1749 / 1763
页数：14
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