A novel fluorescent probe with Aggregation-Induced emission characteristics for PTP1B activity sensing and inhibitor screening

被引：0
|
作者：
Ma, Xiangwei [1 ]
Yang, Zhenzhong [2 ,3 ]
Luo, Yuanlin [2 ]
Jin, Zehua [3 ]
Zou, Jingtao [4 ]
Wang, Yi [2 ,3 ]
Zhao, Xiaoping [5 ]
机构：
[1] Zhejiang Chinese Med Univ, Sch Pharm, Hangzhou 310053, Peoples R China
[2] Zhejiang Univ, Inst Pharmaceut, Coll Pharmaceut Sci, Hangzhou 310058, Peoples R China
[3] Zhejiang Univ, Coll Pharmaceut Sci, State Key Lab Chinese Med Modernizat, Hangzhou 310058, Peoples R China
[4] Tonghua Huaxia Pharmaceut Co, Tonghua 134000, Peoples R China
[5] Zhejiang Chinese Med Univ, Sch Basic Med Sci, Hangzhou 310053, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2025年 / 327卷
基金：
中国国家自然科学基金;
关键词：
Protein tyrosine phosphatase 1B; Aggregation-induced emission; Fluorescent probe; Natural product library; TYROSINE-PHOSPHATASE; 1B; LIGHT-UP PROBE; ASSAY; DISCOVERY;
D O I：
10.1016/j.saa.2024.125394
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Protein tyrosine phosphatase 1B (PTP1B) is an attractive target for the treatment of metabolic diseases such as type 2 diabetes and obesity. In this study, a novel fluorescent probe with aggregation-induced emission (AIE) characteristics was designed and synthesized. Within the fluorescent probe, a tetraphenylethene core is connected to a peptide sequence that can be specifically recognized and hydrolysed by PTP1B. Due to the dephosphorylation of PTP1B, the fluorescent probe exhibited AIE in a turn-on manner, indicating PTP1B activity. This probe was successfully used to detect PTP1B activity in HepG2 cell lysates. Then, a probe-based method was applied to screen for potential PTP1B inhibitors from a natural product library, and three novel PTP1B inhibitors were discovered. These findings indicated that the proposed approach offered a new avenue for discovering potential PTP1B inhibitors.
引用
收藏
页数：9
相关论文
共 50 条
  • [41] A "Turn-on" fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein
    Pan, Xiaoling
    Liu, Pai
    Wu, Xinghui
    Zhang, Yahui
    Cai, Zhengxu
    Shi, Jianbing
    Zhi, Junge
    Li, Zi
    Wang, Dayan
    Tong, Bin
    Dong, Yuping
    SENSORS AND ACTUATORS B-CHEMICAL, 2021, 345
  • [42] A Highly Specific Probe for Sensing Hydrogen Sulfide in Live Cells Based on Copper-Initiated Fluorogen with Aggregation-Induced Emission Characteristics
    Li, Xin
    Yang, Chengyu
    Wu, Kai
    Hu, Yongzhou
    Han, Yifeng
    Liang, Steven H.
    THERANOSTICS, 2014, 4 (12): : 1233 - 1238
  • [43] Discovery of Novel PTP1B Inhibitors by High-throughput Virtual Screening
    Debnath, Abhijit
    Rani, Anjna
    Mazumder, Rupa
    Mazumder, Avijit
    Singh, Rajesh Kumar
    Sharma, Shalini
    Srivastava, Shikha
    Chaudhary, Hema
    Mishra, Rashmi
    Khurana, Navneet
    Sanchitra, Jahanvi
    Jan, Sk Ashif
    CURRENT COMPUTER-AIDED DRUG DESIGN, 2024,
  • [44] Innovative probes with aggregation-induced emission characteristics for sensing gaseous signaling molecules
    Lee, Ka-Wai
    Chen, Huan
    Wan, Yingpeng
    Zhang, Zhen
    Huang, Zhongming
    Li, Shengliang
    Lee, Chun-Sing
    BIOMATERIALS, 2022, 289
  • [45] Novel thiophene derivatives as PTP1B inhibitors with selectivity and cellular activity
    Ye, Deju
    Zhang, Yu
    Wang, Fei
    Zheng, Mingfang
    Zhang, Xu
    Luo, Xiaomin
    Shen, Xu
    Jiang, Hualiang
    Liu, Hong
    BIOORGANIC & MEDICINAL CHEMISTRY, 2010, 18 (05) : 1773 - 1782
  • [46] Hyperbranched Polyester-Based Fluorescent Probe for Histone Deacetylase via Aggregation-Induced Emission
    Yu, Changmin
    Wu, Yinglong
    Zeng, Fang
    Li, Xizhen
    Shi, Jianbin
    Wu, Shuizhu
    BIOMACROMOLECULES, 2013, 14 (12) : 4507 - 4514
  • [47] Instant in situ highlighting of latent fingerprints by a green fluorescent probe based on aggregation-induced emission
    Tian, Rui
    Zhu, Feng-Yu
    Ma, Rongliang
    Wang, Ya-Long
    Huang, Jinliang
    Li, Chong
    Zhu, Ming-Qiang
    BIOSENSORS & BIOELECTRONICS, 2024, 263
  • [48] Synthesis and bioimaging of biodegradable red fluorescent organic nanoparticles with aggregation-induced emission characteristics
    Xu, Dazhuang
    Zou, Hui
    Liu, Meiying
    Tian, Jianwen
    Huang, Hongye
    Wan, Qing
    Dai, Yanfeng
    Wen, Yuanqing
    Zhang, Xiaoyong
    Wei, Yen
    JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2017, 508 : 248 - 253
  • [49] A Novel Sarsolenane Diterpene as a PTP1B Inhibitor from Hainan Soft Coral Sarcophyton trocheliophorum Marenzeller
    Liang, Linfu
    Wang, Jinan
    Shi, Xiaoxuan
    Zhu, Yinghua
    Li, Jia
    Zhu, Weiliang
    Wang, Heyao
    Guo, Yuewei
    CHINESE JOURNAL OF CHEMISTRY, 2017, 35 (08) : 1246 - 1250
  • [50] A highly selective and sensitive fluorescent probe for quantitative detection of Hg2+ based on aggregation-induced emission features
    Wang, Aizhi
    Yang, Yunxu
    Yu, Feifei
    Xue, Lingwei
    Hu, Biwei
    Fan, Weiping
    Dong, Yajun
    TALANTA, 2015, 132 : 864 - 870
12345