Reversible pH-driven conformational switching of tethered superoxide dismutase with gold nanoparticle enhanced surface plasmon resonance spectroscopy

被引：0

作者：

Kang, Taewook ^{[2
]}

Hong, Surin ^{[2
]}

Choi, Inhee ^{[2
]}

Sung, Jung Jun ^{[1
]}

Kim, Younjung ^{[2
]}

Hahn, Ji-Sook ^{[2
]}

Yi, Jongheop ^{[2
]}

机构：

[1] College of Medicine, Institute of Chemical Processes, Seoul National University, San 56-1, Shillim, Kwanak, Seoul 151-742, Korea, Republic of

[2] School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, San 56-1, Shillim, Kwanak, Seoul 151-742, Korea, Republic of

来源：

Journal of the American Chemical Society | 2006年 / 128卷 / 39期

关键词：

A new class of surface-immobilized protein nanomachines can be reversibly actuated by cycling the solution pH between 2.5 and 12.3; which induces a conformational change; thereby modulating the thickness of superoxide dismutase (SOD1) tethered to the Au thin film. By placing Au nanoparticles (AuNP) atop the immobilized SOD1 by means of a gold-thiol assembly; the nanoscale motion of SOD1 at the interface produces mechanical work to lift and then lower the AuNP from the Au substrate by a distance of ca. 3 nm and transduces this motion into an easily measurable reflectivity change in the surface plasmon resonance (SPR) spectrum. As-made supported conjugate consisting of SOD1 and AuNP is quite robust and stable; and its operation in response to pH variations; which mirrors the conformational changes of responsive SOD1 at the interface; is found to be highly reversible and reproducible. This is the first demonstration of the development of novel solid-state sensors and/or switching devices based on substrate-bound protein conformational changes and AuNP enhanced SPR spectroscopy. © 2006 American Chemical Society;

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摘要：

Journal article (JA)

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页码：12870 / 12878