Urea biosensor based on zinc oxide/multi-walled carbon nanotubes/chitosan nanocomposite thin films

被引：0
|
作者：
Verma, Suman [1 ]
Bhanjana, Gaurav [1 ]
Dilbaghi, Neeraj [1 ]
Kumar, Sandeep [1 ]
Umar, Ahmad [2 ,3 ]
机构：
[1] Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar 125001, India
[2] Promising Centre for Sensors and Electronic Devices, Najran University, Najran 11001, Saudi Arabia
[3] Department of Chemistry, College of Science and Arts, Najran University, Najran 11001, Saudi Arabia
来源：
Sensor Letters | 2014年 / 12卷 / 01期
关键词：
Yarn - Biosensors - Chitosan - II-VI semiconductors - Indium compounds - Tin oxides - ITO glass - Cyclic voltammetry - Electrochemical biosensors - Thin films - Enzyme immobilization - Glass substrates - Nanocomposite films - Urea - Carbon films - Fourier transform infrared spectroscopy - Metabolism;
D O I：
10.1166/sl.2014.3241
中图分类号：
学科分类号：
摘要：
For the development of urea biosensor, (zinc oxide-chitosan) ZnO CH and multi-walled carbon nanotubes-chitosan (MWCNT-CH) nano-hybrid films have been developed on indium-tin-oxide (ITO) coated glass substrate. These films act as a bio-molecular matrix for immobilization of urease (Ur) and glutamate dehydrogenase (GLDH). Formation of this nano-hybrid film is confirmed by Fourier transform infrared spectroscopy (FTIR). Both sols are stable against agglomeration as zeta potential value is greater than 30 mV in each case. Cyclic voltammetry (CV) studies show decrease in value of current after the immobilization of urease and glutamate dehydrogenase which indicates insulating properties of urease enzyme. Lower Michaelis-Menten constant (Km) values of ZnO based biosensor (2.1 mg/dL) indicates that this bio-electrode has more enzyme affinity of urease to urea while CNTs based biosensor has better detection limit (0.2 mg/dl) with linear regression 0.998. Copyright © 2014 American Scientific Publishers.
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页码：50 / 55
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