Improve axial resolution of fluorescent microscopy using cylindrical vector beams modulated by diffractive optical elements

被引：0
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作者：
机构：
[1] Optical-Electronic Integration Research Center, China Electronics Technology Group Corporation No.38 Research Institute, Hefei
[2] School of Physical Sciences, University of Science and Technology of China, Hefei
[3] School of Life Sciences, University of Science and Technology of China, Hefei
来源：
Cao, G. (cgw2003@mail.ustc.edu.cn) | 1600年 / Science Press卷 / 40期
关键词：
Axially-selective-activation; Diffractive optics; Super-resolution imaging; Vector beam;
D O I：
10.3788/CJL201340.0410001
中图分类号：
学科分类号：
摘要：
A novel method to improve the axial resolution of fluorescent microscopy using photo-switching characteristics of fluorescent protein is proposed. For microscopy with different numerical apertures, a 100-nm axially-selective-activation is achieved by using cylindrical vector beams with vortex phase as activation and deactivation beams which are modulated by designed diffractive optical elements. Furthermore, adjusting the power ratio R of the activation beam and deactivation beam will make the maximum activation probability of fluorescent protein suitable for single-molecule microscopy. The numerical calculation based on Cy5 demonstrates that along the z axis the activation probability has a single peak with full-width at half-maximum (FWHM) of only 25.7 nm and about 90% of activated molecules are located in a 30-nm-thick layer around zero point (for R=500).
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