Quantum dot conjugated RGD for targeted in vivo imaging of laryngocarcinoma vessel

被引：0

作者：

Zhu, Xiaomei ^{[1
]}

Wang, Xiaomei ^{[2
]}

Feng, Gang ^{[2
]}

Chen, Qiang ^{[1
,2
]}

Lin, Guimiao ^{[2
]}

Zhao, Junting ^{[1
]}

Xu, Gaixia ^{[1
]}

Niu, Hanben ^{[1
]}

机构：

[1] Key Laboratory of The Ministry of Education/Guangdong Optoelectronic Devices and System, College of Optoelectronic Engineering Shenzhen University, Shenzhen

[2] Key Laboratory of Biomedical Engineering of Shenzhen, College of Medicine, Shenzhen University, Shenzhen

来源：

Zhongguo Jiguang/Chinese Journal of Lasers | 2014年 / 41卷 / 05期

关键词：

Cancer blood vessel; Cyclo (Arg-Gly-Asp-D-Phe-Lys) peptides; Medical optics; Quantum dot; Skin fold window chamber;

D O I：

10.3788/CJL201441.0504002

中图分类号：

学科分类号：

摘要：

The targeted imaging of quantum dot (QD) conjugated cyclo (Arg-Gly-Asp-D-Phe-Lys ) peptides [c(RGDfK), QD-RGD] for laryngeal cancer blood vessel in vivo is studied. QD is conjugated with c(RGDfK) peptides by the reaction of carboxyl and amino groups. The spectra stabilities of QD-RGD in RMPI1640 and mouse serum are measured by fluorescence spectrophotometer. The targeting of QD-RGD to αvβ3 on Hep-2 and MCF-7 cells is studied by fluorescent microscope. Finally, the targeting of QD-RGD to laryngeal cancer vascular in dorsal skin fold window chamber by tail intravenous injection is investigated. The result shows that the spectra stability of QD-RGD in RPMI1640 does not obviously change in 4 hours. The fluorescence intensity of QD-RGD in mouse serum in 24 hours only decreases by 20%.The result of cells fluorescence imaging shows that QD-RGD can specifically bind to integrin αvβ3 on cells. The result of vascular imaging shows QD-RGD gathers in cancer blood vessel after injecting for 2 hours, and QD-RGD is removed from cancer blood vessel after 24 hours. The study demonstrates that QD-RGD can be used to targeted image cancer blood vessel in vivo, which offers a reference for studying targeting diagnosis and targeting therapy of laryngocarcinoma in vivo.

引用

共 18 条

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