Multifunctional spatial-potential resolved electrochemiluminescence biosensor for dual-channel simultaneous detection of hepatitis A virus and hepatitis C virus DNA

Highly sensitive simultaneous detection of dual-target is one of the main research directions of electrochemiluminescence (ECL), but the traditional three-electrode system makes it difficult to realize this aim. This work designed a multifunctional spatial-potential resolved ECL biosensor for dual-channel simultaneous detection of hepatitis A virus (HAV) and hepatitis C virus (HCV) DNA. Self-luminescent dual-ligand metal organic framework (D-MOF) and cerium MOF (Ce-MOF) were modified on two parts of the ITO electrode to achieve dualchannel simultaneous detection. One ligand of D-MOF acted as the luminophore and the other as the co-reactant, so D-MOF could emit excellent positive potential ECL signal without requiring external co-reactants. In channel A, the Ag nanoparticles (Ag NPs) quenching probes were introduced to D-MOF through hybridization of HAV with "Y" type DNA. Positive potential signal of D-MOF was decreased for HAV DNA detection based on resonance energy transfer (RET). In channel B, the CdSe quantum dots (QDs) were introduced to Ce-MOF through hybridization of HCV with "Y" type DNA. Due to the excellent ECL performance of CdSe QDs, negative potential signal was increased for HCV DNA detection. In this work, an ingenious spatial-potential resolved ECL biosensor was developed for simultaneous detection of dual-target by simple sealing film isolation, which opened a new strategy for dual-target detection in general ECL assays with three-electrode system.