XVSAP1 from Xerophyta viscosa improves osmotic-, salinity- and high-temperature-stress tolerance in Arabidopsis

被引：19

作者：

Garwe, Dahlia ^{[1
]}

Thomson, Jennifer A. ^{[2
]}

Mundree, Sagadevan G. ^{[2
]}

机构：

[1] Tobacco Research Board, Harare

[2] Department of Molecular and Cell Biology, University of Cape Town, Rondebosch

来源：

Biotechnology Journal | 2006年 / 1卷 / 10期

关键词：

High temperature stress; Osmotic stress; Resurrection plant; Salinity stress; Xerophyta viscosa;

D O I：

10.1002/biot.200600136

中图分类号：

学科分类号：

摘要：

XVSAP1, a gene isolated from a dehydrated Xerophyta viscosa cDNA library, was transformed into Arabidopsis thaliana by Ti plasmid-mediated transformation under the control of a cauliflower mosaic virus 35S promoter, a nos terminator and bar gene selection. Expression of XVSAP1 in Arabidopsis led to constitutive accumulation of the corresponding protein in the leaves. Transgenic Arabidopsis grown in tissue culture maintained higher growth rates during osmotic, high-salinity and high temperature stress, respectively. Non-transgenic plants had shorter roots, leaf expansion was inhibited and leaves were more chlorotic than those of the transgenic plants. This study demonstrates that XVSAP1 has a significant impact on dehydration, salinity and high-temperature stress tolerance in Arabidopsis. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1137 / 1146

页数：9

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