Sequence analysis of alginate-derived oligosaccharides by negative-ion electrospray tandem mass spectrometry

被引：0
|
作者：
Zhang, Zhenqing [1 ]
Yu, Guangli [1 ,4 ]
Zhao, Xia [1 ]
Liu, Haiying [1 ]
Guan, Huashi [1 ]
Lawson, Alexander M. [2 ]
Chai, Wengang [2 ,3 ]
机构：
[1] Institute of Marine Drug and Food, Ocean University of China, Shandong, China
[2] MRC Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Middlesex, United Kingdom
[3] MRC Glycosciences Laboratory, Imperial College London, Northwick Park and St. Mark's Campus, Harrow, Middlesex HA1 3UJ, United Kingdom
[4] Institute of Marine Drug and Food, Ocean University of China, Shandory, 26603, China
来源：
Journal of the American Society for Mass Spectrometry | 2006年 / 17卷 / 04期
关键词：
Negative-ion electrospray tandem mass spectrometry (ES-MS/MS) with collision-induced dissociation (CID) is attempted for sequence determination of alginate oligosaccharides, derived from polyanionic alginic acid, polymannuronate, and polyguluronate by partial depolymerization using either alginate lyase or mild acid hydrolysis. Sixteen homo- and hetero-oligomeric fragments were obtained after fractionation by gel-filtration and strong anion exchange high performance liquid chromatography. The product-ion spectra of these alginate oligosaccharides were dominated by intense B-, C-, Y-, and Z-type ions together with 0,2A- and 2,5A-ions of lower intensities. Internal mannuronate residues (M) produce weak but specific decarboxylated Zint-ions (Zint - 44 Da; int: denotes internal), which can be used for distinction of M and a guluronate residue (G) at an internal position. A reducing terminal M or G, although neither gives rise to a specific ion, can be identified by differences in the intensity ratio of fragment ions of the reducing terminal residue [2,5A red]/[0,4Ared] (red: denotes reducing terminal). © 2006 American Society for Mass Spectrometry;
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页码：621 / 630
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