Dispatched microbial community assembly processes driving ecological succession during phytostabilization of mercury-rich tailings

Phytostabilization is an important way for the remediation of mine tailings, but the associated microbial processes and community succession remain largely unknown. In this study, we investigated the assembly mechanisms maintaining the core and satellite subcommunities diversity during phytostabilizaion of a mercury-rich mine tailings. The contents of total Hg and methylmercury decreased with a concomitant increase of total and available phosphorus content along the successive remediation stages. Microbial community composition, profiled by 16S rRNA gene sequencing, revealed amplicon sequence variants (ASVs) that were separated according to their abundance within either the core community or the satellite community. Community dynamics analysis showed that alpha diversity indices increased for the core community while decreased for the satellite community. Both satellite and core communities were mainly driven by stochastic drift process, and homogeneous selection was relatively higher in shaping the core community organization. The core community included ASVs affiliated to Proteobacteria, Crenarchaeota, Bacteroidota, Verrucomicrobiota, Acidobacteriota, and Myxococcota phyla, which were driven primarily by heterogeneous selection and drift. The satellite community included ASVs affiliated to Acidobacteriota, Ktedonobacteria, Anaerolineae and Verrucomicrobiota phyla, which were mainly influenced by heterogeneous selection. Nineteen taxa and one taxon were identified as keystone taxa for the satellite and core communities respectively. This study provides important insights on the assemble rules within the core and satellite communities, and theoretical guidance for further ecological restoration and management during microbial remediation of metal-mined derelict land.