A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungus Trichoderma reesei

The complete protein pattern of cellulase and hemicellulase genes was studied through the Genome-wide analysis in Trichoderma reesei. The genome database revealed the presence of 39 ORFs encoding related proteins, including 32 enzymes with a catalysis domain related to cellulases and hemicellulases and 7 related proteins with a cellulose-binding module (CBM). Ten of these encoded yet undescribed enzymes, including six novel β-glucosidases or xylosidases, two putative xylanases and two undescribed mannases. To better illustrate the relation of these 39 related proteins, four groups were created and analyzed by phylogenetic analysis: group A corresponding to xylanases, group B belonging to mannases and acting to degrade mannan; group C containing all known and putative cellulose-degrading proteins that have highly conserved CBMs; and group D containing β-glucosidase and β-xylosidase. Group D was the largest group, in which 8 β-glucosidases appeared to be non-secreted proteins. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.