Pancreatic islet cells in microfluidic-spun hydrogel microfibers for the treatment of diabetes

Islet transplantation has been developed as an effective cell therapy strategy to treat the progressive life-threatening disease Type 1 diabetes (TIDM), Te mimic the natural islets and achieve in iso Lation, hydrogel encapsulation of multiple alet cell types is the current endeavor. Here, we present a microfiber loading wah pancreatic a and 18 cells by by microfluidic mich spinning for diabetes treatme nent, Bene fiting from microfluidic technology, the the cells cells could be controllably and continuously loaded in the alginate and methacrylated hyaluronic acid (Alg HAMA) microfiber and maintained their high bioactivity. The re sultant microfiber could then bold the capacity of duad-mode glucose responsareness attributed to the glucagon and immlin secreted by the encapsulated pancreatie a and cells. After transplantation into the brown adipose tissue (BAT), these coll-laden microfibers showed successful blood glarose control in rodents and avoided the occurrence of hypoglycemia. These results conceived that the multicellular microfibers are experteil to to provide new insight into into artificial isle s & aring;et preparation. treanment, and diabetes regenerative medicine as well as tissue engineering Statement of significance The microfibers were generated with a double network of alginate and methacrylated hyaluronic acid. The microfibers could simultaneously encapsulate pancratic a and cells and showed dual-mode glucose responsiveness The cell-laden microfibers maintained a long-term hormone-releasing function in vivo Cell-laden microfibers transplanted diabetic mice could achieve glycemic control for 6 weeks. 2024 The Author(s), Published by Ehevier Lid on behalf of Acta Materialia Inc This is an open access article under the CC IIY license (http://carvecommons.org/levuses/by/4.0/)