Effect of over-expression of V-ATPase subunit C from Antarctic notothenioid fishes on cold tolerance in tobacco

被引：0
作者：
Wang, Tingqin [1 ,2 ]
Zhu, Linghong [1 ]
Peng, Changlian [1 ]
机构：
[1] College Of Life Science, Key Laboratory Of Ecology And Environmental Science In Guangdong Higher Education, Guangdong Provincial Key Lab. Of Biotechnology For Plant Development, South China Normal University
[2] Agricultural College, Guangdong Ocean University
来源：
Biotechnology and Biotechnological Equipment | 2012年 / 26卷 / 06期
关键词：
ATP; Electrolyte leakage activity; Fv/Fm; MDA; pEBV-VIGS; Proline; Soluble protein; Transgenic plants;
D O I：
10.5504/bbeq.2012.0113
中图分类号：
学科分类号：
摘要：
In order to elucidate the function of ATPase subuinit C from Antarctic notothenioid fishes, we designed and adopted a modified pEBV-VIGS (pea early browning virus, pEBV; virus-induced gene silencing, VIGS) virus infection technique (10) to introduce the ATPase gene into tobacco plants. The result of RT-PCR confirmed that the ATPase subuinit C gene was over-expressed in tobacco. In contrast to wild-type plants, these transgenic lines grew faster under low temperature. The physiological and biochemical effects of high-level expression of the pCAPE2-ATP in tobacco were then analyzed; the changes in electrolyte leakage activity, MDA content, proline content, soluble protein content and the ratio of Fv/Fm showed that ATP over-expression in tobacco increased the cold tolerance of plants. These results demonstrated that ATP can possibly be used to enhance the low temperature-tolerance of plants.
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页码：3359 / 3364
页数：5
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