In vitro cytotoxicity evaluation of oxytetracycline loaded cockle shell derived calcium carbonate aragonite nanoparticles

被引：0
|
作者：
Idris S.B. [1 ,5 ]
Arifah A.K. [1 ]
Jesse F.F.A. [2 ]
Ramanoon S.Z. [3 ]
Basit M.A. [1 ,6 ]
Zakaria M.Z.A.B. [1 ,4 ]
机构：
[1] Department of Veterinary Preclinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor
[2] Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor
[3] Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor
[4] Institute of Biosciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, UPM
[5] Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto State
[6] Department of Biosciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan
来源：
Nanoscience and Nanotechnology - Asia | 2021年 / 11卷 / 02期
关键词：
Cytotoxicity; MTT; Nanomedicine; Normal mouse fibroblast; Oxytetracycline; Trypan blue assay;
D O I：
10.2174/2210681210999200420083144
中图分类号：
学科分类号：
摘要：
Background: Evaluation of the toxic effects of nanoparticle-drug in vitro is an important step in the design of new pharmaceutical formulations. Rapid results, reduced cost and easy handling make cell culture models first line in initial toxicological assessment of nano-drug preparations. Objective: To evaluate the in vitro cytotoxicity of oxytetracycline loaded calcium carbonate aragonite nanoparticle in normal mouse fibroblast (NIH3T3) cell line. Methods: NIH3T3 cells were exposed to varying concentrations (6.25-100 µg/mL) of calcium carbonate aragonite nanoparticle (CS-CaCO3NP), oxytetracycline loaded calcium carbonate aragonite nanoparticle (OTC-CS-CaCO3NP) and oxytetracycline (OTC) in 96 well plates for 24, 48 and 72 hours. Cell viability was determined by MTT and trypan blue assays. Results: Both assays showed that CS-CaCO3NP and OTC-CS-CaCO3NP had higher cell viability values compared to OTC. Conclusion: Encapsulating OTC into CS-CaCO3NP reduced its cytotoxicity to NIH3T3 cells using both MTT and trypan blue assay. © 2021 Bentham Science Publishers.
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页码：237 / 242
页数：5
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