In vitro cytotoxicity evaluation of oxytetracycline loaded cockle shell derived calcium carbonate aragonite nanoparticles

被引：0
|
作者：
Idris S.B. [1 ,5 ]
Arifah A.K. [1 ]
Jesse F.F.A. [2 ]
Ramanoon S.Z. [3 ]
Basit M.A. [1 ,6 ]
Zakaria M.Z.A.B. [1 ,4 ]
机构：
[1] Department of Veterinary Preclinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor
[2] Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor
[3] Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor
[4] Institute of Biosciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, UPM
[5] Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto State
[6] Department of Biosciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan
来源：
Nanoscience and Nanotechnology - Asia | 2021年 / 11卷 / 02期
关键词：
Cytotoxicity; MTT; Nanomedicine; Normal mouse fibroblast; Oxytetracycline; Trypan blue assay;
D O I：
10.2174/2210681210999200420083144
中图分类号：
学科分类号：
摘要：
Background: Evaluation of the toxic effects of nanoparticle-drug in vitro is an important step in the design of new pharmaceutical formulations. Rapid results, reduced cost and easy handling make cell culture models first line in initial toxicological assessment of nano-drug preparations. Objective: To evaluate the in vitro cytotoxicity of oxytetracycline loaded calcium carbonate aragonite nanoparticle in normal mouse fibroblast (NIH3T3) cell line. Methods: NIH3T3 cells were exposed to varying concentrations (6.25-100 µg/mL) of calcium carbonate aragonite nanoparticle (CS-CaCO3NP), oxytetracycline loaded calcium carbonate aragonite nanoparticle (OTC-CS-CaCO3NP) and oxytetracycline (OTC) in 96 well plates for 24, 48 and 72 hours. Cell viability was determined by MTT and trypan blue assays. Results: Both assays showed that CS-CaCO3NP and OTC-CS-CaCO3NP had higher cell viability values compared to OTC. Conclusion: Encapsulating OTC into CS-CaCO3NP reduced its cytotoxicity to NIH3T3 cells using both MTT and trypan blue assay. © 2021 Bentham Science Publishers.
引用
收藏
页码：237 / 242
页数：5
相关论文
共 50 条
  • [21] Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles ameliorates lead-induced neurotoxicity in rats via attenuation of oxidative stress
    Mailafiya, Maryam Muhammad
    Abubakar, Kabeer
    Chiroma, Samaila Musa
    Danmaigoro, Abubakar
    Zyoud, Tawfiq Y. T.
    Rahim, Ezamin Bin Abdul
    Moklas, Mohamad Aris Mohd
    Zakaria, Zuki Abu Bakar
    FOOD SCIENCE & NUTRITION, 2023, 11 (05): : 2211 - 2231
  • [22] Development of nanoantibiotic delivery system using cockle shell-derived aragonite nanoparticles for treatment of osteomyelitis
    Saidykhan, Lamin
    Bin Abu Bakar, Md Zuki
    Rukayadi, Yaya
    Kura, Aminu Umar
    Latifah, Aiful Yazan
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2016, 11 : 661 - 673
  • [23] Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles: A novel strategy for the treatment of lead-induced hepato-renal toxicity in rats
    Mailafiya, Maryam Muhammad
    Abubakar, Kabeer
    Chiroma, Samaila Musa
    Danmaigoro, Abubakar
    Rahim, Ezamin Bin Abdul
    Moklas, Mohamad Aris Mohd
    Zakaria, Zuki Abu Bakar
    SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2020, 27 (06) : 1538 - 1552
  • [24] Facile Synthesis of Calcium Carbonate Nanoparticles from Cockle Shells
    Islam, Kh. Nurul
    Zuki, A. B. Z.
    Ali, M. E.
    Bin Hussein, Mohd Zobir
    Noordin, M. M.
    Loqman, M. Y.
    Wahid, H.
    Hakim, M. A.
    Abd Hamid, Sharifa Bee
    JOURNAL OF NANOMATERIALS, 2012, 2012
  • [25] Transformation of amorphous calcium carbonate nanoparticles into aragonite controlled by ACCBP
    Su, Jingtan
    Zhu, Fangjie
    Zhang, Guiyou
    Wang, Hongzhong
    Xie, Liping
    Zhang, Rongqing
    CRYSTENGCOMM, 2016, 18 (12): : 2125 - 2134
  • [26] Antibacterial Activity of Ciprofloxacin-Encapsulated Cockle Shells Calcium Carbonate (Aragonite) Nanoparticles and Its Biocompatability in Macrophage J774A.1
    Isa, Tijani
    Zakaria, Zuki Abu Bakar
    Rukayadi, Yaya
    Hezmee, Mohd Noor Mohd
    Jaji, Alhaji Zubair
    Imam, Mustapha Umar
    Hammadi, Nahidah Ibrahim
    Mahmood, Saffanah Khuder
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (05)
  • [27] In vitro cytotoxicity evaluation of resveratrol-loaded nanoparticles: Focus on the challenges of in vitro methodologies
    Rocha, V.
    Marques, C.
    Figueiredo, J. L.
    Gaio, A. R.
    Costa, P. C.
    Sousa Lobo, J. M.
    Almeida, I. F.
    FOOD AND CHEMICAL TOXICOLOGY, 2017, 103 : 214 - 222
  • [28] Agar and egg shell derived calcium carbonate and calcium hydroxide nanoparticles: Synthesis, characterization and applications
    Khan, Shanza Rauf
    Jamil, Saba
    Rashid, Hummayun
    Ali, Shahid
    Khan, Safyan A.
    Janjua, Muhammad Ramzan Saeed Ashraf
    CHEMICAL PHYSICS LETTERS, 2019, 732
  • [29] Amorphous calcium carbonate: A precursor phase for aragonite in shell disease of the pearl oyster
    Huang, Jingliang
    Liu, Chuang
    Xie, Liping
    Zhang, Rongqing
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 497 (01) : 102 - 107
  • [30] Mollusc larval shell formation: Amorphous calcium carbonate is a precursor phase for aragonite
    Weiss, IM
    Tuross, N
    Addadi, L
    Weiner, S
    JOURNAL OF EXPERIMENTAL ZOOLOGY, 2002, 293 (05): : 478 - 491
12345