Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers

被引：0
|
作者：
Schneider, Robert [1 ]
Ader, Christian [1 ]
Lange, Adam [1 ,3 ]
Giller, Karin [1 ]
Hornig, Sönke [2 ]
Pongs, Olaf [2 ]
Becker, Stefan [1 ]
Baldus, Marc [1 ]
机构：
[1] Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
[2] Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Universität Hamburg, Falkenried 94, 20251 Hamburg, Germany
[3] Laboratory of Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Str. 10, 8093 Zürich, Switzerland
来源：
Journal of the American Chemical Society | 2008年 / 130卷 / 23期
关键词：
We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel; KcsA-Kv1.3; in lipid bilayers. Sequential resonance assignments were obtained using a combination of 15N-13C and 13C-13C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K+ channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function. © 2008 American Chemical Society;
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页码：7427 / 7435
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