Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers

被引：0

作者：

Schneider, Robert ^{[1
]}

Ader, Christian ^{[1
]}

Lange, Adam ^{[1
,3
]}

Giller, Karin ^{[1
]}

Hornig, Sönke ^{[2
]}

Pongs, Olaf ^{[2
]}

Becker, Stefan ^{[1
]}

Baldus, Marc ^{[1
]}

机构：

[1] Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

[2] Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Universität Hamburg, Falkenried 94, 20251 Hamburg, Germany

[3] Laboratory of Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Str. 10, 8093 Zürich, Switzerland

来源：

Journal of the American Chemical Society | 2008年 / 130卷 / 23期

关键词：

We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel; KcsA-Kv1.3; in lipid bilayers. Sequential resonance assignments were obtained using a combination of 15N-13C and 13C-13C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K+ channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function. © 2008 American Chemical Society;

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学科分类号：

摘要：

Journal article (JA)

引用

页码：7427 / 7435

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